Anti-VAMP1 Monoclonal Antibody for WB - P23763

Core Product Specifications and Parameters

Biological Background: VAMP1 Function and Localization

• VAMP1 (vesicle-associated membrane protein 1), also known as synaptobrevin-1, is a member of the SNARE protein family involved in vesicle docking and fusion.
• It plays a critical role in the targeting and fusion of transport vesicles to their target membrane, essential for neurotransmitter release at synapses.
• The gene undergoes alternative splicing, yielding isoforms that may exhibit distinct functional properties.
• Subcellular localization includes cytoplasmic vesicle membrane, synaptic vesicle membrane, synaptosome, and mitochondrial outer membrane, reflecting participation in both secretory and mitochondrial pathways.
• Tissue expression is prominent in the nervous system, skeletal muscle, and adipose tissue.
• Post-translational modifications such as phosphorylation and the presence of a coiled-coil domain are key for SNARE complex formation and regulation.
• Mutations are linked to congenital myasthenic syndrome (CMS25) and neurodegenerative conditions, underscoring its physiological importance.
• The protein has a small molecular weight of ~13 kDa (12902 Da), typical of vesicle SNAREs.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 1–118 amino acids; for specificity validation, consider peptide blocking or pre-absorption with the recombinant protein.
• Western blot: anticipate a band at ~13 kDa. Use a 0.2 µm PVDF membrane and optimize transfer conditions to retain small proteins.
• Immunofluorescence/ICC: VAMP1 is membrane-bound; validate co-localization with synaptic (e.g., synaptophysin) or mitochondrial markers, and choose permeabilization method carefully.
• Immunohistochemistry: optimal antigen retrieval may require testing both heat- and enzyme-based protocols due to the transmembrane topology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-VAMP1 monoclonal antibody—ideal for neuroscience, synaptic biology, and neuromuscular disease research. We supply validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and can source rare-target raw materials to streamline your assay development pipeline. From concept to clinic, CamelBio bridges your procurement needs with reliable, high-quality products.