Anti-β1-Adrenergic Receptor Rabbit Polyclonal Antibody for WB, ELISA - P08588

Core Product Specifications and Parameters

Biological Background: Beta-1 Adrenergic Receptor Function and Localization

• Beta-1 adrenergic receptor (ADRB1, also known as beta-1 adrenoreceptor) is a member of the G protein-coupled receptor (GPCR) family, encoded by the ADRB1 gene.

• It functions as a receptor for catecholamines (epinephrine and norepinephrine), coupling primarily to G(s) proteins to activate adenylate cyclase and downstream cAMP signaling pathways. Related references: PMID:10212248, PMID:12391161, PMID:15358775

• The receptor binds epinephrine and norepinephrine with approximately equal affinity, mediating catecholamine-induced physiological responses. Related references: PMID:33093660

• Through cAMP-dependent interaction with RAPGEF2, ADRB1 activates the Ras signaling pathway, linking to cell proliferation and differentiation. Related references: PMID:12391161

• In the heart, ADRB1 plays a critical role in the sympathetic nervous system regulation of cardiac function, including positive chronotropic and inotropic effects via stimulation of cardiomyocyte contraction. Related references: PMID:11052857

• ADRB1 is also involved in the central regulation of sleep/wake behaviors, likely through catecholaminergic signaling in the brain. Related references: PMID:11854867, PMID:31473062

• Subcellularly, ADRB1 is a multi-pass transmembrane protein localized at the cell membrane, where it undergoes post-translational modifications including disulfide bond formation, glycosylation, palmitoylation, and phosphorylation, which are critical for receptor trafficking and signaling dynamics.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C-terminal intracellular domain (amino acids 350-450) of human ADRB1. This region is chosen to avoid cross-reactivity with other adrenergic receptor subtypes. For WB, consider using whole-cell lysates or membrane preparations, and validate using appropriate positive controls.
• The predicted molecular weight is approximately 51 kDa, but due to glycosylation and other modifications, the observed band may appear at a higher molecular weight. For ELISA, the antibody may be used for both direct and indirect detection; however, specific assay conditions require optimization.
• Cross-reactivity with mouse and rat ADRB1 has been reported, but sequence homology should be verified, and performance should be validated in the specific sample system and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop procurement solution for IVD raw materials, including validated antibodies, reagents, and technical support from concept to clinic. This anti-β1-adrenergic receptor antibody supports cardiovascular and neurological disease research, and we can source additional related materials such as antibody pairs, bulk ancillary reagents, and rare target raw materials to streamline your assay development.