Anti-β-TrCP/BTRC Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y297

Core Product Specifications and Parameters

Biological Background: β-TrCP/BTRC Function and Localization

• Protein Identity and SCF Complex Assembly: β-TrCP/BTRC (F-box/WD repeat-containing protein 1A) is the substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. It specifically binds to phosphorylated target proteins, mediating their ubiquitination and subsequent proteasomal degradation. Related references: PMID:10066435, PMID:10497169, PMID:10644755
• NF-κB Pathway Regulation: SCF(BTRC) ubiquitinates phosphorylated NFKBIA (IκBα), NFKBIB, and NFKBIE, leading to their degradation and allowing NF-κB nuclear translocation and transcription activation. This is critical for immune and inflammatory responses. Related references: PMID:10066435, PMID:10497169, PMID:10644755, PMID:10835356
• Wnt/β-Catenin Signaling: It mediates ubiquitination of CTNNB1 (β-catenin), a key effector in the Wnt pathway, thereby controlling its protein stability and downstream gene expression. Related references: PMID:12077367, PMID:12820959
• Circadian Clock Control: BTRC is essential for the clock-dependent transcription rhythm by targeting phosphorylated PER1 and PER2 for proteasomal degradation. Related references: PMID:15917222
• Other Key Targets: SCF(BTRC) ubiquitinates a broad range of substrates including CDC25A (cell cycle), SNAI1 (EMT), DEPTOR, TFE3, MITF (mTORC1 signaling), and GLI2/GLI3 (Hedgehog pathway), implicating it in diverse cellular processes. Related references: PMID:10835356, PMID:14603323, PMID:22017875, PMID:22017876, PMID:22017877, PMID:16705181, PMID:16371461
• Subcellular Localization: β-TrCP/BTRC localizes to both the cytoplasm and the nucleus, consistent with its role in targeting substrates in different compartments.
• Tissue Expression and Structural Features: Widely expressed with high levels in epididymis at the protein level. The protein contains an F-box domain and multiple WD repeats that facilitate substrate recruitment and complex formation; alternative splicing generates isoforms, and the protein is implicated in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal 1-120 amino acid region of human β-TrCP/BTRC. When using this antibody in other species (mouse, rat), verify epitope conservation via sequence alignment.
• For Western blot (WB), the predicted molecular weight is 69 kDa; use appropriate positive control lysates (e.g., HeLa, HEK293) and consider treatment with proteasome inhibitors to stabilize target proteins.
• In IHC-P and IF/ICC, optimize fixation and permeabilization conditions, as the protein is present in both nucleus and cytoplasm. Include blocking steps to reduce background.
• For ELISA, the antibody can be paired with a detection antibody to quantify endogenous or recombinant β-TrCP/BTRC; pilot testing with recombinant protein is recommended.
• Avoid repeated freeze-thaw cycles; aliquot and store at -20°C or below as recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw materials, offering a comprehensive range of antibodies, antigen pairs, and ancillary reagents. This β-TrCP/BTRC polyclonal antibody is available alongside validated monoclonal alternatives, recombinant detection antibodies, and bulk custom supplies, enabling seamless integration into diagnostic assays, particularly in ubiquitin-proteasome and signaling pathway research. Let CamelBio bridge your raw-material gaps and accelerate time-to-market with reliable, cost-effective solutions.