Anti-β-TrCP/BTRC Rabbit mAb for WB, IHC-P, ELISA - Q9Y297

Core Product Specifications and Parameters

Biological Background: β-TrCP/BTRC Function and Localization

• F-box/WD repeat-containing protein 1A (also known as E3RSIkappaB, beta-TrCP) functions as a substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex, mediating ubiquitination and proteasomal degradation of target proteins by recognizing phosphorylated substrates. Related references: PMID:10066435, PMID:10497169, PMID:10644755

• SCF(BTRC) mediates ubiquitination of CTNNB1 to participate in Wnt signaling and ubiquitinates NFKBIA, NFKBIB, and NFKBIE to regulate NF-kappa-B activation; it also targets the p105 form of NFKB1 for degradation. Related references: PMID:12077367, PMID:12820959, PMID:10835356, PMID:11158290, PMID:14673179

• Ubiquitination of CEP68 by SCF(BTRC) is required for centriole separation during mitosis. Related references: PMID:25503564, PMID:25704143

• SCF(BTRC) controls clock-dependent transcription by degrading phosphorylated PER1 and PER2. Related references: PMID:15917222

• Acts as a negative regulator of smoothened signaling by mediating ubiquitination of GLI2 and GLI3, promoting their processing into transcription repressors. Related references: PMID:16705181, PMID:16371461

• Mediates ubiquitination and degradation of additional substrates including REST, mTORC1 regulators (DEPTOR, TFE3, MITF), and UBR2 in T-cell receptor signaling. Related references: PMID:18354482, PMID:21258371, PMID:22017875, PMID:22017876, PMID:22017877, PMID:38225265

• Localizes to both the cytoplasm and nucleus. Tissue expression is reported in epididymis at the protein level. Keywords include host-virus interaction, alternative splicing, WD repeats, and Wnt signaling pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human BTRC. Consider this epitope location when designing blocking or competition experiments.
• For Western blot, the predicted molecular weight is 69 kDa. Include appropriate loading controls and validate band specificity in your sample system.
• Phosphorylation-dependent interactions are central to BTRC function; using phosphatase inhibitors during sample preparation may help preserve physiologically relevant complexes.
• In IHC-P, test antigen retrieval conditions and confirm staining with appropriate tissue controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities to diagnostic manufacturers and research laboratories. This β-TrCP/BTRC antibody supports studies in ubiquitination, Wnt signaling, and NF-kappa-B pathways, complementing CamelBio's extensive portfolio for oncology and signal transduction research. For customized or large-scale sourcing needs, our technical team is ready to assist.