Anti-β-arrestin1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P49407

Core Product Specifications and Parameters

Biological Background: β-arrestin1 Function and Localization

• β-arrestin-1 (ARRB1/ARB1/ARR1) is a member of the arrestin family and is also known as arrestin beta-1 or non-visual arrestin-2.
• It mediates GPCR desensitization and resensitization, playing a central role in terminating G protein signaling and promoting receptor internalization. Related references: PMID:37209686 PMID:38175886 PMID:40384633
• As a scaffold protein, it assembles signaling complexes that activate MAPK (ERK1/2) and AKT pathways, often from endosomal compartments.
• It negatively regulates NOTCH1 signaling by recruiting the ubiquitin ligase ITCH, leading to NOTCH1 ubiquitination and degradation. Related references: PMID:23886940
• It participates in MRGPRE-mediated metabolic signaling, promoting ALDOA phosphorylation, glycolysis, and GLP-1 secretion, thereby improving glucose tolerance. Related references: PMID:40446798
• Subcellularly, β-arrestin-1 is found in the cytoplasm, nucleus, at the plasma membrane, in clathrin-coated pits, pseudopodia, and cytoplasmic vesicles, reflecting its dynamic trafficking.
• Post-translational modifications include phosphorylation and ubiquitination, which modulate its activity and interactions (Keywords: Phosphoprotein, Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 169-418 of human β-arrestin1, encompassing the C-terminal domain that mediates receptor interactions and scaffolding functions.
• For Western blot, detect a band around 47 kDa in human, mouse, or rat cell lysates; validate using appropriate positive controls (e.g., cells expressing high levels of GPCRs).
• In immunofluorescence (IF/ICC), β-arrestin1 shows cytoplasmic and nuclear localization under basal conditions; agonist stimulation may induce translocation to the plasma membrane or endosomes, requiring fixation optimization.
• For immunoprecipitation (IP), use cell lysates from transfected or endogenous sources; the antibody may co-immunoprecipitate interaction partners such as GPCRs or signaling kinases.
• In ELISA applications, consider coating with recombinant β-arrestin1 or peptide immunogen; validate with purified protein standards.
• Cross-reactivity with mouse and rat is reported; however, sequence homology suggests potential reactivity in other species; verify in target species.

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CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated polyclonal antibodies like this anti-β-arrestin1 product. Our portfolio covers a wide range of GPCR signaling targets, along with ancillary reagents and custom sourcing services, ensuring a streamlined supply chain from early-stage research to clinical applications.