Anti-β-arrestin1 Rabbit Monoclonal Antibody for WB and ELISA - P49407

Core Product Specifications and Parameters

Biological Background: β-arrestin1 Function and Localization

• β-arrestin-1 regulates agonist-mediated GPCR signaling by mediating receptor desensitization and resensitization processes. Related references: PMID:37209686 PMID:38175886 PMID:40384633
• It also functions as an endocytic adapter, targeting many receptors for internalization via clathrin-coated pits, and can act as a signaling scaffold for MAPK, AKT1, and p38 pathways.
• Upon GPCR activation, β-arrestin-1 can translocate to the nucleus and regulate transcription by recruiting histone acetyltransferases to promoter regions.
• It negatively regulates the NOTCH signaling pathway by mediating ITCH-dependent ubiquitination and degradation of NOTCH1. Related references: PMID:23886940
• It participates in MRGPRE-mediated signaling to improve glucose tolerance by promoting ALDOA phosphorylation, increasing glycolysis and GLP-1 secretion. Related references: PMID:40446798
• The protein is localized in the cytoplasm, nucleus, cell membrane, clathrin-coated pits, pseudopodia, and cytoplasmic vesicles, reflecting its dynamic role in receptor trafficking.
• β-Arrestin-1 is subject to phosphorylation and ubiquitination, and it exists in alternatively spliced isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human β-arrestin1, which is conserved in mouse; consider using this antibody for detecting endogenous β-arrestin1 in human and mouse cell lysates by Western blot.
• For Western blot, whole-cell lysates or tissue homogenates can be prepared using standard RIPA buffer; validate the antibody in the relevant sample system as expression levels and post-translational modifications may affect detection.
• The antibody is suitable for ELISA; optimize coating antigen concentration and detection parameters for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibodies like this anti-β-arrestin1 monoclonal antibody for GPCR signaling research. We offer comprehensive sourcing solutions—from optimized monoclonal/polyclonal antibodies and bulk ancillary reagents to rare target raw-material procurement—to support your assay development and production needs.