Anti-β-Amyloid(1-42) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, DB, ELISA - P05067

Core Product Specifications and Parameters

Biological Background: β-Amyloid(1-42) Function and Localization

• Amyloid-beta precursor protein (APP) functions as a cell surface receptor involved in neurite growth, neuronal adhesion, axonogenesis, and synaptogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis. Related references: PMID:25122912

• APP participates in cell mobility and transcription regulation, inhibits Notch signaling, and couples to apoptosis-inducing pathways. It acts as a kinesin I membrane receptor, mediating axonal transport of beta-secretase and presenilin 1. Related references: PMID:17062754 PMID:23011729

• The protein plays a critical role in copper homeostasis and oxidative stress; copper-metallated APP can induce neuronal death. It regulates neurite outgrowth via interactions with extracellular matrix components such as heparin and collagen.

• Amyloid-beta peptides, including the 1-42 variant, are lipophilic metal chelators that bind copper, zinc, and iron. They inhibit metal-catalyzed oxidation of lipoproteins and promote tau aggregation and phosphorylation. β-Amyloid(1-42) is a more effective reductant than the 1-40 form and may activate mononuclear phagocytes, eliciting inflammatory responses.

• Subcellular locations encompass the cell membrane, perikaryon, growth cone, clathrin-coated pit, early endosome, endoplasmic reticulum, Golgi apparatus, secreted fractions, cell surface, nucleus, and cytoplasm, reflecting its diverse trafficking and functional roles.

• Expressed predominantly in the brain and cerebrospinal fluid, with highest levels in the frontal lobe cortex. Isoform APP695 is the major neuronal form, while other isoforms are found in non-neuronal cells and T-lymphocytes. Related references: PMID:2649245

• Key biological themes from UniProt keywords include Alzheimer disease, amyloidosis, apoptosis, cell adhesion, neurodegeneration, Notch signaling, and metal binding, underscoring its central role in neurodegenerative pathology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 691–770 of human APP, a region that encompasses the β-amyloid sequence. For Western blot detection of the cleaved 4 kDa peptide, consider optimizing gel percentage and transfer conditions to retain small proteins.
• Given the antibody’s cross-reactivity with mouse, it can be employed in transgenic mouse models of Alzheimer's disease. Validate in the relevant sample system to confirm specificity.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), antigen retrieval and fixation methods should be empirically determined to preserve epitope integrity.
• In ELISA and dot blot (DB) applications, use appropriate peptide controls to ensure accurate quantification of β-amyloid(1-42) levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers a comprehensive portfolio of IVD raw materials, including this β-Amyloid(1-42) antibody, to accelerate Alzheimer’s disease diagnostic development. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, ensuring a reliable supply chain from concept to clinical application. Our team also specializes in sourcing rare targets, offering tailored support to bridge your research and manufacturing needs.