Anti-β-Amyloid(1-40) Rabbit Monoclonal Antibody for IF-P, IHC-P, DB, ELISA - P05067

Core Product Specifications and Parameters

Biological Background: β-Amyloid(1-40) / APP Function and Localization

• APP is a type I transmembrane protein that functions as a cell surface receptor, promoting neurite outgrowth, neuronal adhesion, and synaptogenesis. Interaction between APP molecules on adjacent cells facilitates synapse formation. Related references: PMID:25122912
• Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1, and plays a role in anterograde transport of cargo towards synapses. Related references: PMID:17062754, PMID:23011729
• Proteolytic processing of APP generates amyloid-beta peptides, including β-Amyloid(1-40) and (1-42), which are lipophilic metal chelators with metal-reducing activity, binding transient metals like copper, zinc, and iron. These peptides can inhibit metal-catalyzed oxidation of lipoproteins.
• β-Amyloid(1-42) is a more effective reductant than β-Amyloid(1-40) and may activate mononuclear phagocytes, eliciting inflammatory responses in the brain.
• The gamma-CTF peptides and caspase-cleaved C-terminal fragments, such as C31, are potent enhancers of neuronal apoptosis.
• APP is expressed in the brain and cerebrospinal fluid, with the neuronal isoform APP695 predominant in the CNS, while APP751 and APP770 are widely expressed in non-neuronal cells.
• Subcellularly, APP localizes to the cell membrane, growth cones, clathrin-coated pits, early endosomes, endoplasmic reticulum, Golgi, and is also secreted; amyloid-beta peptides are found in the extracellular space and CSF.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 671-770 of human APP (NP_000475.1), a region encompassing the C-terminal portion of the amyloid-beta peptide and beyond. This antibody may recognize full-length APP, C-terminal fragments, and the free β-Amyloid(1-40) peptide; validate specificity in your experimental system.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), consider optimizing fixation and permeabilization conditions. Antigen retrieval may be necessary for paraffin-embedded tissues.
• In dot blot (DB) and ELISA applications, ensure the peptide is properly coated or immobilized. The antibody’s performance may depend on the conformation and purity of the peptide sample.
• Cross-reactivity with mouse enables testing in mouse models of Alzheimer's disease; however, confirm reactivity in your specific strain and tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing bridge for IVD raw materials, offering diagnostic developers access to validated monoclonal antibodies, antibody pairs, and ancillary reagents. For Alzheimer's disease and amyloid research, we provide anti-β-Amyloid antibodies and support materials tailored for assay development. Contact our team to explore custom sourcing solutions that accelerate your project from concept to clinic.