Anti-β-Amyloid Monoclonal Antibody for IF-P, IHC-P, DB, ELISA - P05067

Core Product Specifications and Parameters

Biological Background: Amyloid-beta Precursor Protein (APP/β-Amyloid) Function and Localization

• APP is a transmembrane precursor protein that yields amyloid-beta peptides; multiple isoforms arise via alternative splicing, with APP695 predominant in neurons and APP751/APP770 in non-neuronal tissues.
• Homophilic interactions between APP molecules on neighboring cells promote synaptogenesis, contributing to synapse formation and plasticity. Related references: PMID:25122912
• APP acts as a kinesin-1 membrane receptor, mediating the anterograde axonal transport of beta-secretase and presenilin 1 toward synapses. Related references: PMID:17062754, PMID:23011729
• APP and its derived amyloid-beta peptides are involved in metal homeostasis, binding copper, zinc, and iron; copper-metallated APP induces oxidative stress and neuronal death, while amyloid-beta peptides inhibit metal-catalyzed lipoprotein oxidation.
• The protein localizes to the cell membrane, clathrin-coated pits, early endosomes, Golgi apparatus, endoplasmic reticulum, and is secreted; in neurons, it concentrates in growth cones and the perikaryon.
• APP is highly expressed in the brain (especially frontal and perisylvian cortices), kidney, heart, and spleen; its aberrant processing leads to amyloid-beta accumulation, a hallmark of Alzheimer's disease.

Experimental Guidance and Technical Tips

• Given the synthetic peptide immunogen, the epitope likely resides within the amyloid-beta sequence; consider using amyloid-beta peptide competition assays to confirm specificity.
• For IHC-P, standard heat-induced antigen retrieval in citrate buffer (pH 6.0) is recommended; optimize incubation times based on tissue type and fixation.
• This antibody cross-reacts with mouse APP; it may be suitable for staining in mouse Alzheimer’s disease models, but validate performance in your specific model system.
• In ELISA applications, coat plates with recombinant APP or synthetic peptide and titrate the antibody to determine optimal working concentration.
• For dot blot (DB), apply serial dilutions of purified amyloid-beta peptides to assess sensitivity and linear range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to a comprehensive portfolio of IVD raw materials. Our β-Amyloid monoclonal antibody is part of a broader offering including optimized antibody pairs, ancillary reagents, and custom sourcing for rare targets. With expertise spanning Alzheimer’s biomarker discovery and assay development, CamelBio supports your journey from concept to clinic.