Anti-UVRAG Polyclonal Antibody for WB, IHC-P, ELISA - Q9P2Y5

Core Product Specifications and Parameters

Biological Background: UVRAG Function and Localization

• UVRAG (UV radiation resistance-associated gene protein), also known as p63, is a versatile regulator of membrane trafficking, including COPI-dependent retrograde transport from Golgi and autophagy, by associating with the NRZ complex and PI3K complex II. Related references: PMID:16799551, PMID:18552835, PMID:20643123, PMID:24056303
• It activates the lipid kinase activity of PIK3C3 and is essential for autophagosome maturation, endocytosis, and ATG9A trafficking. Related references: PMID:16799551, PMID:24056303
• UVRAG promotes late endocytic membrane fusion by enhancing class C Vps/HOPS complex association with SNAREs and facilitating Rab7 activation. Related references: PMID:18552835, PMID:28306502, PMID:24550300
• In the nucleus, UVRAG maintains chromosomal stability by promoting DNA double-strand break repair through non-homologous end joining (NHEJ) via interaction with the DNA-PK complex. Related references: PMID:22542840
• It localizes to multiple subcellular compartments including late endosomes, lysosomes, autophagosomes, early endosomes, endoplasmic reticulum, midbody, and centromeres.
• Highly expressed in brain, lung, kidney, and liver, highlighting its importance in these tissues.
• UVRAG contains a coiled coil domain and undergoes phosphorylation; it is involved in viral entry for negative-strand RNA viruses by promoting endocytic transport and VAMP8-specific SNARE assembly. Related references: PMID:24550300

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using 30–50 µg of total protein lysate from human, mouse, or rat cell lines or tissues, given the predicted molecular weight of ~78 kDa.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; validate in appropriate positive control tissues such as brain or liver where UVRAG is highly expressed.
• The immunogen corresponds to amino acids 501–699 of human UVRAG; ensure that the detected band aligns with the expected size and appears in control samples.
• Cross-reactivity is reported for human, mouse, and rat; for other species, sequence homology analysis is recommended.
• This polyclonal antibody may be suitable for ELISA under optimized conditions; perform titration to determine the optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality antibodies like this anti-UVRAG polyclonal antibody. Targeting UVRAG, a protein central to autophagy, membrane trafficking, and DNA repair, this product supports research in neurodegenerative diseases, cancer, and infectious disease entry mechanisms. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.