Anti-UTP18 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y5J1

Core Product Specifications and Parameters

Biological Background: UTP18 Function and Localization

• UTP18 (also known as WDR50 or CGI-48) is the human homolog of the yeast U3 small nucleolar RNA-associated protein 18, a key component of the small subunit (SSU) processome.
• As part of the SSU processome, UTP18 participates in the early stages of ribosome biogenesis, facilitating the assembly, folding, modification, and processing of the nascent pre-ribosomal RNA.
• UTP18 is specifically involved in the nucleolar processing of pre-18S ribosomal RNA, an essential step for the maturation of the small 40S ribosomal subunit.
• The protein localizes to the nucleus, particularly the nucleolus, consistent with its role in ribosome biogenesis.
• Belonging to the WD repeat protein family, UTP18 features multiple WD40 domains that mediate protein-protein interactions within the processome.
• Post-translational modifications include phosphorylation and isopeptide bond formation, which may regulate its function in response to cellular signals.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein encompassing amino acids 70-260, which may detect full-length UTP18 as well as any truncated variants containing this region.
• For Western blot, it is advisable to optimize antibody dilution empirically; typical starting dilutions range from 1:500 to 1:2000 for chemiluminescent detection.
• Given UTP18's nuclear localization, preparing nuclear-enriched fractions may improve signal intensity in WB.
• This antibody has been tested with human and mouse samples; cross-reactivity with other species should be verified experimentally.
• For ELISA applications, consider direct coating with the immunogen or cell lysates and validate with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For ribosome biogenesis and nucleolar research, we can source rare-target antibodies like UTP18 and provide bulk quantities to support your diagnostic development. Partner with us for tailored solutions from concept to clinic.