Anti-USP9X Polyclonal Antibody for WB, ELISA - Q93008

Core Product Specifications and Parameters

Biological Background: USP9X Function and Localization

• USP9X encodes a deubiquitinase that processes ubiquitin precursors and removes ubiquitin from proteins, modulating protein turnover and preventing degradation.
  Related references: PMID:18254724 PMID:19135894 PMID:22371489
• The enzyme selectively hydrolyzes Lys-11-, Lys-63-, Lys-48-, and Lys-6-linked polyubiquitin chains.
  Related references: PMID:30914461
• USP9X is an essential component of TGF-β/BMP signaling, deubiquitinating monoubiquitinated SMAD4, opposing the E3 ligase TRIM33.
  Related references: PMID:19135894
• It stabilizes RICTOR by removing Lys-63-linked polyubiquitin chains, promoting mTORC2 complex assembly and kinase activity.
  Related references: PMID:33378666
• USP9X regulates chromosome alignment and segregation during mitosis by controlling BIRC5/survivin localization to centromeres.
  Related references: PMID:16322459
• It enhances circadian clock function by deubiquitinating and stabilizing BMAL1.
  Related references: PMID:29626158
• Subcellularly, USP9X resides in the cytosol, growth cones, and cilium axoneme, consistent with roles in neuronal growth and ciliary function.
• The protein is widely expressed in embryonic and adult tissues and is implicated in intellectual disability (XLID99) when mutated.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide corresponding to amino acids 1–100 of human USP9X. Consider using Western blot (WB) or ELISA as primary detection methods; always validate antibody performance in your specific sample system.
• USP9X is a high molecular weight protein (~290 kDa). For WB, use a low-percentage polyacrylamide gel (e.g., 6–8%) and prolonged transfer times to ensure efficient detection.
• Cross-reactivity is reported with Human, Mouse, and Rat; however, verify species compatibility in your experimental context.
• The antibody is supplied as a rabbit polyclonal; therefore, batch-to-batch consistency should be evaluated for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, from concept to clinic. This USP9X polyclonal antibody is part of our extensive portfolio supporting deubiquitinase research and diagnostic development. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to meet your project needs.