Anti-USP8 Rabbit pAb for WB, IF/ICC, ELISA - P40818

Core Product Specifications and Parameters

Biological Background: USP8 Function and Localization

• USP8, also known as ubiquitin carboxyl-terminal hydrolase 8, is a deubiquitinating enzyme that removes conjugated ubiquitin from substrate proteins, thereby regulating protein stability and turnover.
• It hydrolyzes both Lys-48- and Lys-63-linked polyubiquitin chains, and its catalytic activity is enhanced during M phase, linking it to cell cycle regulation (Keywords: Cell cycle, Phosphoprotein).
• USP8 plays a critical role in endosomal sorting and membrane traffic by controlling ubiquitin dynamics on early endosomes. It deubiquitinates EPS15 and maintains ESCRT-0 stability, thus preserving endosome morphology.
• In growth factor signaling, USP8 removes ubiquitin from the epidermal growth factor receptor (EGFR), modulating receptor degradation and downstream MAPK pathway activity.
• USP8 deubiquitinates BACE1, inhibiting its lysosomal degradation and affecting amyloid precursor protein (APP) processing and amyloid-beta formation. Related references: PMID:27302062
• Additionally, USP8 is involved in T-cell anergy regulation via complex formation with RNF128 and OTUB1, and contributes to acrosome biogenesis during spermatogenesis.
• Subcellularly, USP8 localizes to the cytoplasm, nucleus, endosome membrane, and cell membrane, reflecting its diverse functional roles.
• Mutations in USP8 are associated with Cushing syndrome and other disorders, highlighting its clinical significance (Keywords: Disease variant, Cushing syndrome).

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-270) of human USP8, which may recognize multiple isoforms due to alternative splicing. Verify isoform specificity in your experimental system.
• For Western blot, expect a major band around 128 kDa under reducing conditions. Validate the observed molecular weight against known controls and consider using a low percentage gel for optimal separation of high-molecular-weight proteins.
• In IF/ICC, USP8 displays dynamic subcellular localization. Include appropriate permeabilization and blocking steps; co-staining with organelle markers (e.g., EEA1 for early endosomes) is recommended.
• For ELISA, the antibody can be used as a capture or detection reagent, but optimal concentrations must be determined empirically for each assay format.
• This antibody shows confirmed cross-reactivity with mouse and rat USP8. When working with rodent models, always include species-matched positive and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This USP8 polyclonal antibody is part of our comprehensive portfolio for ubiquitin-proteasome system research and cell signaling pathway studies. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and diagnostic programs.