Anti-USP53 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q70EK8

Core Product Specifications and Parameters

Biological Background: USP53 Function and Localization

• USP53 is a deubiquitinase that specifically cleaves 'Lys-63'-linked polyubiquitin chains from substrate proteins, requiring at least three ubiquitin molecules for activity and performing en bloc cleavage. Related references: PMID:32124521 PMID:39587316
• USP53 modulates tight junction barrier properties and mechanical stability by deubiquitinating tight junction proteins such as MARVELD2 and LSR. Related references: PMID:32124521 PMID:39587316
• The deubiquitinase activity of USP53 is essential for the survival of auditory hair cells and normal hearing. Related references: PMID:32124521 PMID:39587316
• USP53 is localized at tight junctions, where it plays a role in cell junction integrity.
• Predominantly expressed in skeletal muscle and heart.
• Mutations in USP53 are associated with intrahepatic cholestasis and deafness, highlighting its clinical relevance.
• USP53 is a zinc-dependent hydrolase belonging to the ubiquitin-specific peptidase family.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 495-582 within the central region of human USP53. Consider the potential for recognizing multiple isoforms or processed forms if present in your sample.
• For Western blotting, a band around 120 kDa is expected. Validate detection in human and mouse lysates from tissues such as skeletal muscle or heart, where USP53 is highly expressed.
• In immunofluorescence applications, USP53 should localize to tight junctions; consider co-staining with junctional markers to confirm specificity.
• For ELISA, ensure the recombinant protein or peptide antigen is appropriately coated, and titrate the antibody to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw material sourcing, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-USP53 antibody serves as a reliable tool for studying ubiquitin signaling and tight junction biology. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to streamline your assay development.