Anti-USP48 Rabbit Polyclonal Antibody for WB, ELISA - Q86UV5

Core Product Specifications and Parameters

Biological Background: USP48 Function and Localization

• Ubiquitin carboxyl-terminal hydrolase: processes polyubiquitin precursors and ubiquitinated proteins. Related references: PMID:16214042 PMID:34059922
• Regulates NF-kappa-B pathway through interaction with RELA and TRAF2.
• Stabilizes Aurora B kinase during cell cycle progression. Related references: PMID:34445214
• In H. pylori infection, stabilizes RELA to dampen apoptosis via TNFAIP3. Related references: PMID:35913642
• Modulates ciliary and synaptic transport, stabilizing ARL3 and UNC119 for photoreceptor health. Related references: PMID:36293380
• Promotes pyroptosis by stabilizing gasdermin E (GSDME). Related references: PMID:36607699
• Localizes to cytoplasm, nucleus, and cilium.
• Widely expressed; fetal inner ear expression links to non-syndromic deafness. Related references: PMID:34059922

Experimental Guidance and Technical Tips

• The immunogen is an N-terminal recombinant fragment (aa 1–300); the antibody likely recognizes conserved USP48 domains across human, mouse, and rat.
• For Western blot, prepare whole cell lysates from USP48-expressing cells; the predicted band is ~119 kDa. Validate with appropriate positive and negative controls.
• For ELISA, consider using recombinant USP48 protein or peptide as coating antigen; optimize antibody dilution empirically.
• Since USP48 is involved in multiple pathways, include relevant pathway inhibitors or siRNAs in experiments to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for USP48 research. We support diagnostic manufacturers and labs with sourcing and technical consulting for deubiquitinase-related targets and signaling pathways. Partner with CamelBio for reliable supply and expertise from concept to clinic.