Anti-USP47 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q96K76

Core Product Specifications and Parameters

Biological Background: USP47 Function and Localization

• USP47 (Ubiquitin carboxyl-terminal hydrolase 47), also known as TRFP, is a deubiquitinating enzyme belonging to the ubiquitin-specific protease family.
• It functions as a thiol protease that specifically cleaves monoubiquitin from DNA polymerase beta (POLB), thereby stabilizing POLB and contributing to base-excision repair (BER) processes.
• USP47 acts as a regulator of cell growth and genome integrity, playing a critical role in maintaining genomic stability.
• It may indirectly regulate the expression of CDC25A at the transcriptional level, implicating USP47 in cell cycle control.
• USP47 is predominantly localized in the cytoplasm, consistent with its role in cytoplasmic signaling and protein homeostasis.
• Tissue expression is prominent in skeletal muscle, heart, and testis, suggesting specialized functions in these tissues.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its enzymatic activity and interactions.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 1128-1287) of human USP47. This region may confer specific recognition; consider sequence homology when evaluating cross-reactivity.
• For Western blot (WB), a band at approximately 157 kDa is expected. Use an appropriate gel percentage (e.g., 6-8% SDS-PAGE) and optimize transfer conditions for high-molecular-weight proteins.
• Validate the antibody in lysates from cell lines expressing USP47, such as HeLa, or tissues like skeletal muscle, heart, and testis, where USP47 is abundantly expressed.
• For immunohistochemistry (IHC-P), antigen retrieval and dilution may require optimization; include positive and negative controls relevant to the sample.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic development and research. This USP47 antibody is suitable for investigations into DNA repair, genome integrity, and cell cycle regulation. For rare target sourcing or custom requirements, our team provides dedicated procurement and technical logistics to streamline your workflow.