Anti-USP4 Polyclonal Antibody for WB, IF/ICC, ELISA - Q13107

Core Product Specifications and Parameters

Biological Background: USP4 Function and Localization

• USP4 (ubiquitin-specific protease 4), also designated UNP or Unph, is a deubiquitinating enzyme that removes ubiquitin from target proteins, participating in diverse cellular processes.
• It deubiquitinates multiple substrates including PDPK1, TRIM21, ADORA2A, HAS2, RHEB, and MAVS, thereby modulating signaling cascades such as mTORC1, receptor trafficking, and innate immunity. Related references: PMID:16316627, PMID:16339847, PMID:22347420, PMID:28604766, PMID:30514904, PMID:39589880
• USP4 enhances antiviral innate immune responses by deubiquitinating MAVS, which increases type I interferon production. Related references: PMID:39589880
• It may regulate pre-mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3, potentially affecting U4/U6.U5 tri-snRNP stability. Related references: PMID:20595234
• The protein is localized to both the cytoplasm and nucleus.
• USP4 is overexpressed in small cell lung tumors and adenocarcinomas, and is also expressed in hippocampal neurons.
• It is classified as a hydrolase, thiol protease, proto-oncogene, and is involved in ubiquitin conjugation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 560–700 within the C-terminal region of human USP4, a region conserved across relevant isoforms. Consider verifying reactivity in your specific sample type or application.
• In Western blotting, a predominant band near 109 kDa is expected under standard reducing conditions. For IF/ICC, appropriate fixation and permeabilization methods should be optimized.
• This polyclonal antibody has been applied in WB, IF/ICC, and ELISA. When using for new assays or modifications, titration and validation in the relevant experimental system are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies validated raw materials for IVD development, including optimized polyclonal antibodies like this anti-USP4 reagent, monoclonal antibody pairs, and bulk ancillary reagents. For projects targeting deubiquitinases, post-translational modifications, or immune-related biomarkers, our one-stop channel streamlines procurement from concept to clinic. Contact us for support in sourcing rare-target or custom-format raw materials.