Anti-USP39 Rabbit Polyclonal Antibody for WB and ELISA - Q53GS9

Core Product Specifications and Parameters

Biological Background: USP39 Function and Localization

• USP39 is a ubiquitin carboxyl-terminal hydrolase (deubiquitinating enzyme) containing a zinc-finger domain, belonging to the peptidase C19 family; it is also known as SAD1 homolog and U4/U6.U5 tri-snRNP-associated 65 kDa protein (SNRNP65).
• Plays a central role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP complex, essential for spliceosome assembly. Related references: PMID:11350945, PMID:26912367
• Deubiquitinates and stabilizes CHK2, thereby regulating the G2/M cell cycle checkpoint and apoptosis in response to DNA damage. Related references: PMID:30771428
• Facilitates DNA repair by promoting recruitment of XRCC4/LIG4 to DNA double-strand breaks for non-homologous end-joining. Related references: PMID:34614178
• Enhances antiviral innate immunity by stabilizing STAT1 and reducing its Lys-6-linked ubiquitination, thus sustaining type I IFN signaling. Related references: PMID:33127822
• Acts as a negative regulator of NF-κB signaling by deubiquitinating and stabilizing NFKBIA (IκBα). Related references: PMID:36651806
• Regulates AURKB mRNA levels and plays a role in cytokinesis and spindle checkpoint control. Related references: PMID:18728397
• Specifically regulates immunoglobulin gene rearrangement in a spliceosome-dependent manner, which is essential for B-cell development.
• Localizes predominantly in the nucleus, consistent with its functions in splicing, DNA repair, and signal transduction.

Experimental Guidance and Technical Tips

• This antibody was produced using a recombinant immunogen corresponding to amino acids 336–565 of human USP39; it is expected to recognize the full-length ~65 kDa protein.
• Validated applications include Western blot (WB) and ELISA; optimal working dilutions should be determined empirically in the end-user’s experimental system.
• Cross-reactivity is reported for human, mouse, and rat; consider sequence alignment for other species if needed.
• Given USP39's nuclear localization, nuclear fractionation or whole-cell lysates from tissues/cell lines with high USP39 expression (e.g., HeLa, HEK293) are recommended for WB detection.
• The anticipated molecular weight is approximately 65 kDa under reducing conditions; note that post-translational modifications may cause slight variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials and technical services, from early development to clinical application. This anti-USP39 polyclonal antibody is part of our extensive portfolio for splicing and DNA damage research; we also provide antibody pairs, bulk reagents, and custom sourcing for rare targets. Let us streamline your supply chain with reliable, one-stop procurement solutions tailored to your assay development needs.