Anti-USP39 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q53GS9

Core Product Specifications and Parameters

Biological Background: USP39 Function and Localization

• Ubiquitin carboxyl-terminal hydrolase 39 (USP39), also known as SAD1 homolog and U4/U6.U5 tri-snRNP-associated 65 kDa protein, is a deubiquitinating enzyme containing a zinc-finger motif.
• Essential component of the U4/U6-U5 tri-snRNP complex, playing a critical role in pre-mRNA splicing and spliceosome assembly. Related references: PMID:11350945 PMID:26912367
• Regulates AURKB mRNA levels, thereby contributing to cytokinesis and the spindle checkpoint during cell division. Related references: PMID:18728397
• Functions in DNA damage response by deubiquitinating and stabilizing CHK2, regulating apoptosis and G2/M cell cycle arrest. Related references: PMID:30771428
• Facilitates DNA double-strand break repair by controlling XRCC4/LIG4 recruitment for non-homologous end-joining. Related references: PMID:34614178
• Exhibits antiviral activity through stabilization of STAT1 and modulation of type I IFN signaling. Related references: PMID:33127822
• Negative regulator of NF-kappa-B activation by deubiquitinating and stabilizing NFKBIA. Related references: PMID:36651806
• Localized in the nucleus, USP39 is a phosphoprotein involved in alternative splicing, ubiquitin conjugation, and metal-binding pathways.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide within amino acids 200-300 of human USP39. Consider this epitope region when designing complementary reagents or performing validation studies.
• For Western blotting (WB), anticipate a band at approximately 65 kDa. Validate detection in human and rat lysates, as the antibody shows cross-reactivity with both species.
• In immunohistochemistry (IHC-P), nuclear staining is expected due to USP39’s subcellular localization. Antigen retrieval methods may be required; optimize according to tissue type.
• ELISA protocols can be developed with standard direct or sandwich formats; verify linearity and sensitivity using recombinant USP39 or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined, one-stop platform for IVD raw-material procurement. From validated antibody pairs and optimized monoclonal antibodies like this anti-USP39 rabbit mAb to bulk ancillary reagents and challenging-target sourcing, we support every step of your development pipeline. Whether you need robust splicing or DNA repair markers, or rare specificity raw materials, CamelBio’s integrated supply chain and technical consultancy help accelerate your transition from concept to clinic.