Anti-USP33 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8TEY7

Core Product Specifications and Parameters

Biological Background: USP33 Function and Localization

• USP33, also known as VDU1, is a deubiquitinating enzyme that cleaves ubiquitin from substrate proteins, thereby regulating their stability, signaling, and intracellular trafficking.
• It is essential for centrosome duplication, stabilizing the centriolar protein CCP110 through deubiquitination during the S and G2/M phases of the cell cycle.
• USP33 regulates Slit/ROBO1-mediated cell migration and axon guidance by deubiquitinating the ROBO1 receptor, critical for commissural axon crossing in the neural tube.
• It modulates GPCR signaling by deubiquitinating beta-arrestins (ARRB1/2) and the beta-2 adrenergic receptor (ADRB2), controlling receptor recycling and resensitization after agonist stimulation.
• The enzyme contributes to thyroid hormone metabolism by deubiquitinating and stabilizing DIO2, the activating deiodinase.
• USP33 is capable of removing both Lys-48- and Lys-63-linked polyubiquitin chains, indicating a broad role in ubiquitin signaling.
• It is localized to the cytoplasm, perinuclear region, centrosome, and Golgi apparatus, and is widely expressed across tissues.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band at approximately 107 kDa; use appropriate loading controls and validate specificity with siRNA or knockout samples if available.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may require optimization; consider testing a range of antibody dilutions on control tissues.
• For immunofluorescence (IF/ICC), include appropriate fixation and permeabilization steps suited for centrosomal or Golgi localization studies.
• This polyclonal antibody may exhibit lot-to-lot variation; for consistent results in quantitative assays, consider using the same batch throughout an experiment.
• Cross-reactivity with mouse and rat orthologs is expected based on sequence homology, but validation in the specific model system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material procurement, offering this anti-USP33 polyclonal antibody to support research in deubiquitination, centrosome biology, and GPCR signaling. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, streamlining sourcing for diagnostic manufacturers and laboratories. From concept to clinic, CamelBio bridges your research needs with reliable, one-stop access to rare-target raw materials and technical expertise.