Anti-USP30 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q70CQ3

Core Product Specifications and Parameters

Biological Background: USP30 Function and Localization

• USP30 (Ubiquitin carboxyl-terminal hydrolase 30) is a deubiquitinating enzyme also known as deubiquitinating enzyme 30, ubiquitin thioesterase 30, and ubiquitin-specific-processing protease 30, encoded by the USP30 gene.
• Tethered to the mitochondrial outer membrane, USP30 acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN); it hydrolyzes ubiquitin attached by parkin on target proteins such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Related references: PMID:18287522 PMID:24896179 PMID:25527291
• Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, two types of linkage involved in mitophagic signaling; does not efficiently cleave polyubiquitin phosphorylated at 'Ser-65'. Related references: PMID:25621951 PMID:25527291
• Negatively regulates mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2.
• Subcellular location: mitochondrion outer membrane; contains a transmembrane helix.
• Tissue specificity: expressed in skeletal muscle, pancreas, liver, and kidney.
• Belongs to the peptidase C19 family; classified as a thiol protease and hydrolase involved in the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 57-390 of human USP30, covering the catalytic core; consider this region's conservation when assessing reactivity in mouse and rat.
• For Western blotting, a band around 59 kDa is expected; validate the detected band size in your specific sample system as post-translational modifications may shift migration.
• In immunofluorescence/ICC, mitochondrial outer membrane staining should be anticipated; counterstaining with a mitochondrial marker is recommended to confirm localization.
• For ELISA applications, optimize coating conditions and antibody dilutions using a recombinant USP30 protein standard if available.
• When studying mitophagy regulation, consider concomitant pharmacological or genetic manipulation of parkin to evaluate USP30 deubiquitinase activity in your model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, streamlining the path from discovery to clinical application. Our offerings include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For mitochondrial quality control and ubiquitin pathway research, we provide reliable reagents to accelerate your assay development and biomarker studies.