Anti-USP25 (USP21) Rabbit Polyclonal Antibody for WB, ELISA - Q9UHP3

Core Product Specifications and Parameters

Biological Background: USP25 Function and Localization

• USP25 is a deubiquitinating enzyme that hydrolyzes ubiquitin moieties from substrate proteins, playing critical roles in inflammation and immune response. Related references: PMID:29518389, PMID:37683630
• It modulates the Wnt/β-catenin pathway by deubiquitinating and stabilizing tankyrases TNKS1 and TNKS2. Related references: PMID:28619731, PMID:30926243, PMID:38875478
• USP25 regulates the KEAP1-NRF2 axis in oxidative stress defense by deubiquitinating KEAP1, leading to NRF2 degradation. Related references: PMID:37339955
• Positively regulates RNA virus-induced innate signaling by deubiquitinating ERLIN1 and ERLIN2, restricting virus production via cholesterol biosynthetic flux. Related references: PMID:37683630
• Acts as a negative regulator of IL-17-mediated signaling and inflammation by removing Lys-63-linked ubiquitination of TRAF5 and TRAF6. Related references: PMID:23042150
• Prevents ubiquitination and degradation of TRAF3 to reduce JNK and P38 phosphorylation, IL-1B secretion, and induce endotoxin tolerance. Related references: PMID:30579117
• USP25 localizes to both the cytoplasm and the nucleus.
• The muscle-specific isoform USP25m may regulate muscular differentiation, while USP25a is highly expressed in the brain (cerebral cortex) and USP25b is found in most tissues except heart and skeletal muscle. Related references: PMID:38875478

Experimental Guidance and Technical Tips

• The immunogen, an N-terminal fragment (aa 1-230), may recognize multiple USP25 isoforms. In WB, consider using lysates from testis, pancreas, or brain where USP25a is abundant.
• For ELISA, validate coating with recombinant protein or peptide to determine optimal antibody dilution.
• When analyzing mouse or rat samples, include appropriate controls to confirm expected cross-reactivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted sourcing partner for high-quality IVD antibodies and reagents, including this anti-USP25 polyclonal antibody for research into ubiquitin pathways, inflammation, and cell signaling. In addition to validated antibodies, we offer bulk ancillary reagents, optimized antibody pairs, and rare target raw-material procurement to streamline your diagnostic development from concept to clinic.