Anti-USP25 Rabbit Monoclonal Antibody for WB - Q9UHP3

Core Product Specifications and Parameters

Biological Background: USP25 Function and Localization

• USP25, also known as ubiquitin carboxyl-terminal hydrolase 25 or deubiquitinating enzyme 25, is a member of the ubiquitin-specific-processing protease family. Multiple isoforms exist, including the widely expressed USP25a, ubiquitously distributed USP25b, and muscle-specific USP25m.
• Functions as a deubiquitinating enzyme that hydrolyzes ubiquitin from substrates, playing critical roles in inflammation and immune responses. Related references: PMID:29518389, PMID:37683630
• Modulates the Wnt/β-catenin signaling pathway through deubiquitination and stabilization of tankyrases TNKS1 and TNKS2. Related references: PMID:28619731, PMID:30926243, PMID:38875478
• Regulates KEAP1-NRF2 axis in oxidative stress defense by deubiquitinating KEAP1, leading to NRF2 degradation. Related references: PMID:37339955
• Positively regulates RNA virus-induced innate immune signaling by deubiquitinating ERLIN1 and ERLIN2, thereby restricting virus production via modulation of cholesterol biosynthesis. Related references: PMID:37683630
• Acts as a negative regulator of interleukin-17 (IL-17)-mediated signaling and inflammation by removing Lys-63-linked ubiquitination from TRAF5 and TRAF6. Related references: PMID:23042150
• Prevents ubiquitination and degradation of TRAF3, reducing JNK and P38 phosphorylation, IL-1B secretion, and contributing to endotoxin tolerance. Related references: PMID:30579117
• Localizes to both the cytoplasm and nucleus, consistent with its roles in signaling and transcriptional regulation.
• The muscle-specific isoform USP25m may be involved in regulation of muscular differentiation and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-170) of human USP25, which may target conserved domains across isoforms.
• Validated applications include Western blotting (WB), immunoprecipitation (IP), immunohistochemistry of paraffin-embedded tissues (IHC-P), and ELISA. Users should optimize antibody dilution for each application in their specific assay conditions.
• The antibody cross-reacts with human, mouse, and rat USP25, enabling multi-species studies.
• Due to reported protein weights of 122, 125, and 129 kDa, users may observe multiple bands corresponding to isoforms; validate with appropriate controls.
• For functional studies involving ubiquitination pathways, consider using this antibody in combination with ubiquitin-specific inhibitors and appropriate deubiquitinase assay buffers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting for every stage from concept to clinic. This anti-USP25 rabbit monoclonal antibody supports research in ubiquitin-mediated signaling, inflammation, and cancer, reflecting our commitment to supplying high-quality deubiquitinase antibodies. Beyond this product, CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your assay development and production needs.