Anti-USP24 Rabbit Polyclonal Antibody for WB, ELISA - Q9UPU5

Core Product Specifications and Parameters

Biological Background: USP24 Function and Localization

• USP24, also known as ubiquitin-specific-processing protease 24 or deubiquitinating enzyme 24, is a ~294 kDa member of the ubiquitin-specific protease (USP) family.
• It functions as a deubiquitinase, cleaving ubiquitin from protein substrates, thereby regulating protein stability and cellular processes.
• USP24 modulates cell survival by controlling the stability of key proteins, including DDB2, MCL1, and TP53.
• Plays a positive role in ferritinophagy, the selective autophagic degradation of ferritin, contributing to iron homeostasis.
• Contains a catalytic thiol protease domain typical of USPs and is subject to phosphorylation that may regulate its activity.
• Interacts with viral proteins, implicating a role in host-virus interactions (based on keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 2431–2620) of human USP24; this unique sequence may confer specificity to the antibody.
• For Western blot, consider optimizing antibody dilution and incubation conditions using a suitable positive control; due to the large size of USP24 (~294 kDa), ensure adequate transfer conditions (e.g., extended transfer time or low methanol percentage).
• For ELISA, recombinant USP24 protein or the immunogen peptide can be used for coating; titration of both coating antigen and antibody is recommended.
• Given the reported cross-reactivity with human and mouse, validate reactivity in relevant sample systems if switching between species.
• As a polyclonal antibody, it may recognize multiple epitopes, which can enhance detection sensitivity but may also require careful assessment of specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality antibodies and ancillary reagents. This anti-USP24 rabbit polyclonal antibody is suitable for investigations into ubiquitin pathways, cell survival, and iron metabolism. As a one-stop sourcing partner, CamelBio can also supply validated antibody pairs, bulk ancillary reagents, and assist in sourcing rare-target raw materials to streamline your IVD development.