Anti-USP22 Rabbit Monoclonal Antibody for WB and ELISA - Q9UPT9

Core Product Specifications and Parameters

Biological Background: USP22 Function and Localization

• USP22 is a ubiquitin carboxyl-terminal hydrolase (deubiquitinase) also known as USP3L, encoded by the USP22 gene (KIAA1063, USP3L).
• As part of the SAGA histone acetyltransferase complex, USP22 catalyzes the deubiquitination of histones H2A and H2B, functioning as a transcriptional coactivator and being recruited by MYC to gene promoters. Related references: PMID:18206972, PMID:18206973, PMID:18469533
• USP22 promotes cell cycle progression by stabilizing cyclin B1 (CCNB1) and protecting it from proteasome-mediated degradation. Related references: PMID:27030811
• Through stabilization of the deacetylase SIRT1, USP22 antagonizes TP53 transcriptional activation, thereby modulating cell cycle and apoptosis. Related references: PMID:22542455
• USP22 participates in antiviral innate immunity by deubiquitinating KPNA2, leading to IRF3 nuclear translocation and type I interferon production, and also negatively regulates STING1 to control type III IFN signaling. Related references: PMID:32130408, PMID:35933402
• It stabilizes CD274 (PD-L1) to maintain immune tolerance and controls necroptotic cell death via regulation of RIPK3 phosphorylation and ubiquitination. Related references: PMID:31399419, PMID:33369872
• USP22 localizes to both the nucleus and cytoplasm; it is moderately expressed in heart and skeletal muscle, with weaker expression in lung and liver.

Experimental Guidance and Technical Tips

• The immunogen (N-terminal amino acids 1-175) is well suited for detecting full-length USP22; consider using nuclear and cytoplasmic extracts to verify subcellular localization in WB.
• For Western blot, the predicted molecular weight is approximately 60 kDa; include appropriate positive controls and validate in your sample of interest.
• In ELISA applications, ensure that the coating antigen or capture antibody conditions are optimized; titrate the antibody to determine the optimal working concentration for your system.
• Cross-reactivity with mouse USP22 has been confirmed; additional species reactivity should be validated individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For USP22-related research in transcriptional regulation, cell cycle, and innate immunity, we supply validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our global sourcing network can also assist in obtaining rare deubiquitinase targets and custom reagents to streamline your assay development.