Anti-USP22 Polyclonal Antibody for WB, ELISA - Q9UPT9

Core Product Specifications and Parameters

Biological Background: USP22 Function and Localization

• Protein identity and isoforms: USP22 (Ubiquitin carboxyl-terminal hydrolase 22), also known as deubiquitinating enzyme 22, ubiquitin thioesterase 22, or USP3L, is a member of the ubiquitin-specific processing protease family.

• Core nucleosome/chromatin function: As a component of the SAGA histone acetylation complex, USP22 catalyzes the deubiquitination of histones H2A and H2B, functioning as a transcriptional coactivator. Related references: PMID:18206972 PMID:18206973 PMID:18469533

• Cell cycle and apoptosis regulation: USP22 facilitates cell-cycle progression by stabilizing CCNB1, preventing its proteasome-mediated degradation. It also modulates apoptosis by stabilizing SIRT1, which deacetylates and represses TP53 transcriptional activity. Related references: PMID:27030811 PMID:22542455

• Subcellular localization: USP22 localizes to both the nucleus and cytoplasm, consistent with its roles in transcriptional regulation and cytoplasmic signaling.

• Roles in immunity and inflammation: USP22 participates in antiviral responses by deubiquitinating KPNA2 to promote IRF3 nuclear translocation and type I interferon production. It negatively regulates STING1 activation in type III IFN signaling. Additionally, it inhibits NLRP3 inflammasome activation via autophagy-dependent NLRP3 degradation, stabilizes CD274 to maintain immune tolerance, and controls necroptosis by regulating RIPK3 phosphorylation. Related references: PMID:32130408 PMID:35933402 PMID:31399419 PMID:33369872

• Antimicrobial and pro-inflammatory roles: During bacterial infection, USP22 promotes pro-inflammatory responses by removing Lys-48-linked polyubiquitination from TRAF6.

• Key post-translational modifications and keywords: USP22 undergoes acetylation and phosphorylation. It is a zinc-dependent thiol protease containing a zinc-finger domain.

• Tissue specificity: USP22 is moderately expressed in heart and skeletal muscle, and weakly expressed in lung and liver.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide corresponding to amino acids 50–150 of human USP22 (NP_056091.1). Researchers may consider validating the antibody’s reactivity with mouse USP22, given the reported cross-reactivity.
• This antibody is recommended for Western blot (WB) and ELISA applications. For WB, a band around 60 kDa is expected based on the target’s molecular weight. Optimize loading controls and detection conditions within the relevant sample system.
• For ELISA, consider titrating the antibody concentration to achieve optimal signal-to-noise ratio, and validate with appropriate positive and negative controls.
• As USP22 is involved in dynamic cellular processes, treat samples carefully to preserve deubiquitinase activity and modifications if studying functional states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw material sourcing, connecting diagnostic manufacturers and research laboratories with high-quality antibodies and ancillary reagents. For USP22 research—which spans transcriptional regulation, cell cycle control, and immune signaling—CamelBio offers reliable procurement of validated polyclonal antibodies, optimized monoclonal options, and the capability to source rare-target reagents in bulk. Complementing the product supply, our technical consultation supports your development from concept to clinic.