Anti-USP21 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UK80

Core Product Specifications and Parameters

Biological Background: USP21 Function and Localization

• USP21 (Ubiquitin carboxyl-terminal hydrolase 21) is a deubiquitinating enzyme belonging to the USP family, also known as USP16, USP23, and deubiquitinating enzyme 21.
• It deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a transcriptional coactivator. Deubiquitination of H2A relieves repression of H3K4 di- and trimethylation, regulating transcriptional initiation (By similarity).
• USP21 deubiquitinates BAZ2A/TIP5, leading to its stabilization and involvement in epigenetic regulation. Related references: PMID:26100909
• It can remove NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Related references: PMID:10799498
• USP21 acts as a negative regulator of ribosome quality control (RQC) by mediating deubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS20/uS10, thereby antagonizing ZNF598-mediated 40S ubiquitination. Related references: PMID:32011234
• Subcellular localization includes both cytoplasm and nucleus.
• Highly expressed in heart, pancreas, and skeletal muscle, with lower expression in brain, placenta, liver, kidney, and very low in lung.
• Key features include thiol protease activity, zinc-binding, chromatin regulation, and involvement in the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the N-terminal region (amino acids 1–100) of human USP21. For best results, validate reactivity in your specific sample system due to potential epitope masking or post-translational modifications.
• This polyclonal antibody is suitable for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA applications. Consider running appropriate positive and negative controls, and optimize working dilutions for each assay.
• Reported species cross-reactivity includes human, mouse, and rat. Perform additional validation if using in other species, as epitope conservation may vary.
• The predicted protein weight is 63 kDa; however, actual migration may differ due to post-translational modifications or isoform variations. Use a molecular weight marker for accurate band identification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents, to support your diagnostic and research workflows. This USP21 rabbit polyclonal antibody is part of our extensive catalog tailored for epigenetic and ubiquitin-pathway studies. Our team can assist with sourcing rare targets and providing technical consultation to accelerate your project from concept to clinic.