Anti-USP15 Monoclonal Antibody for WB, IP, ELISA - Q9Y4E8

Core Product Specifications and Parameters

Biological Background: USP15 Function and Localization

• USP15 (ubiquitin carboxyl-terminal hydrolase 15), also known as UNPH4 or UNPH-2, is a deubiquitinating enzyme that cleaves ubiquitin chains from target proteins, and is expressed as multiple isoforms with observed molecular weights of ~27 kDa and 109–112 kDa due to alternative splicing.
• Functions as a critical regulator of TGF-beta receptor signaling by deubiquitinating monoubiquitinated R-SMADs (SMAD1/2/3) or stabilizing TGFBR1, thereby promoting TGF-beta target gene activation. Related references: PMID:21947082, PMID:22344298
• Modulates the NF-kappa-B pathway by mediating deubiquitination of NFKBIA within the COP9 signalosome complex, linking it to transcriptional regulation. Related references: PMID:16005295, PMID:17318178, PMID:19576224
• Acts as an inhibitor of mitophagy by counteracting parkin (PRKN)-mediated ubiquitination; hydrolyzes Lys-48- and Lys-63-linked polyubiquitin chains on mitochondrial substrates such as MFN2. Related references: PMID:24852371
• Deubiquitinates histone H2B, thereby influencing gene expression and DNA repair processes. Related references: PMID:24526689
• Participates in endosome organization by deubiquitinating SQSTM1/p62, promoting vesicle transport to the cell periphery. Related references: PMID:27368102
• Negatively regulates antifungal immunity through Lys-27-linked deubiquitination of CARD9, leading to its inactivation. Related references: PMID:33093067
• Localizes to the cytoplasm, nucleus, and mitochondrion, and is predominantly expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (amino acids 968–981) of human USP15, designed for specific recognition of both full-length and shorter isoforms.
• For Western blotting, expect predominant bands at ~27 kDa and 109–112 kDa; validate detection in the relevant sample system and optimize blocking/dilution conditions.
• This antibody is suitable for immunoprecipitation; consider using cell or tissue lysates expressing USP15 and confirm co-precipitation with known interaction partners.
• In ELISA applications, carefully titrate the coating antigen and antibody concentration to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. This anti-USP15 monoclonal antibody targets the ubiquitin-proteasome system and TGF‑β/mitophagy research areas. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and production needs.