Anti-USP14 Polyclonal Antibody for WB, ELISA - P54578

Core Product Specifications and Parameters

Biological Background: USP14 Function and Localization

• USP14, also known as ubiquitin carboxyl-terminal hydrolase 14 or deubiquitinating enzyme 14, is a proteasome-associated deubiquitinase (DUB) that recycles ubiquitin from proteasomal substrates (PubMed:18162577, 28396413). Related references: PMID:18162577, PMID:28396413
• It exists in both proteasome-bound and free cytoplasmic pools, and its deubiquitinating activity is critical for maintaining free ubiquitin levels in cells (PubMed:18162577).
• USP14 is required for the degradation of CXCR4 chemokine receptor, impacting CXCL12-mediated chemotaxis (PubMed:19106094). Related references: PMID:19106094
• Under non-stressed conditions, USP14 acts as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) by interacting with ERN1 to prevent degradation of unfolded ER proteins (PubMed:19135427). Related references: PMID:19135427
• It plays an indispensable role in synaptic development and function at neuromuscular junctions (By similarity).
• USP14 contributes to innate immune defense by stabilizing the viral DNA sensor CGAS, thereby inhibiting its autophagic degradation (PubMed:27666593). Related references: PMID:27666593
• As a negative regulator, USP14 inhibits OPTN-mediated selective autophagy of KDM4D, modulating H3K9me2/me3 levels (PubMed:35145029). Related references: PMID:35145029
• Subcellular localization: predominantly cytoplasmic, with a fraction associated with the cell membrane (UniProt).

Experimental Guidance and Technical Tips

• The immunogen peptide is located within residues 400-500 of human USP14 (NP_005142.1). Consider this domain when assessing potential isoform or species cross-reactivity.
• This polyclonal antibody has been validated for Western blot (WB) and ELISA. For other applications such as immunohistochemistry or co-immunoprecipitation, additional optimization and validation are recommended.
• USP14 expression and proteasome association can vary across cell types and conditions; appropriate sample processing (e.g., cell fractionation) may improve detection sensitivity.
• As a deubiquitinase, USP14 activity may be influenced by proteasome inhibitors (e.g., MG-132) or DUB inhibitors; coordinate experiments accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including high-quality antibodies for ubiquitin-proteasome research. Our USP14 antibody is part of a broader portfolio of deubiquitinase and proteasome-related reagents, complemented by technical and sourcing support from concept to clinic. Contact us for bulk orders, custom antibody services, and tailored solutions to accelerate your assay development.