Anti-USP13 Rabbit Polyclonal Antibody for WB, ELISA - Q92995

Core Product Specifications and Parameters

Biological Background: USP13 Function and Localization

• USP13, also known as Isopeptidase T-3 (ISOT-3) or Ubiquitin carboxyl-terminal hydrolase 13, is a deubiquitinase that removes ubiquitin from target proteins, regulating processes such as autophagy, ERAD, cell cycle progression, and DNA damage response. Related references: PMID:21571647 PMID:32772043 PMID:33592542
• It is a key component of a regulatory loop controlling autophagy by deubiquitinating and stabilizing BECN1 and VPS34, thereby promoting autophagosome formation. Related references: PMID:32101753
• USP13 participates in endoplasmic reticulum-associated degradation (ERAD) by deubiquitinating SKP2 and UBL4A, ensuring proper protein quality control in the ER.
• It regulates cell cycle progression by stabilizing cell cycle proteins such as SKP2 and AURKB. Related references: PMID:32772043
• The protein is involved in the DNA damage response and maintenance of genomic stability via deubiquitination of RAP80 and TOPBP1. Related references: PMID:33592542
• USP13 positively regulates type I and type II interferon signaling by deubiquitinating STAT1, but negatively regulates antiviral response via STING1 deubiquitination. Related references: PMID:23940278 PMID:28534493
• Subcellularly, USP13 localizes to the cytoplasm.
• It is highly expressed in ovary and testes, and contains zinc-finger domains and a thiol protease active site.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 90–200 of human USP13, a region within the N-terminal portion of the protein. This design may offer specificity for USP13 over other USP family members.
• For western blot (WB), a band at approximately 97 kDa is expected. Validate the antibody in relevant cell or tissue lysates, and consider using protease inhibitors during sample preparation to preserve USP13 detection.
• This antibody shows cross-reactivity with mouse and rat, enabling its use in rodent model studies. Perform a pilot experiment to confirm reactivity in your specific sample type.
• For ELISA, optimize the coating antigen concentration and antibody dilution to achieve the desired signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, providing high-quality antibodies like this anti-USP13 polyclonal antibody to support research in autophagy, cell cycle regulation, and DNA damage response. We offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare-target raw materials to streamline your assay development workflow. Contact us to discuss your specific procurement needs.