Anti-USP12 Polyclonal Antibody for WB, ELISA - O75317

Core Product Specifications and Parameters

Biological Background: USP12 Function and Localization

• USP12 is a deubiquitinating enzyme that localizes to the nucleus, cytoplasm, and cell membrane.
• Regulates immune response and inflammation by deubiquitinating key signaling proteins. Related references: PMID:19075014, PMID:27373336
• Stabilizes the TCR signaling complex by deubiquitinating LAT and TRAT1 upon T-cell receptor engagement. Related references: PMID:26811477
• Essential for LPS-induced macrophage response through activation of the NF-κB pathway. Related references: PMID:28063927
• Promotes antiviral immune response by targeting DNA sensor IFI16 to inhibit its proteasomal degradation. Related references: PMID:37410794
• Modulates interferon signaling independently of deubiquitinase activity by maintaining nuclear phosphorylated STAT1 levels. Related references: PMID:31899788
• Negative regulator of NOTCH signaling by deubiquitinating non-activated NOTCH receptors, targeting them for lysosomal degradation. Related references: PMID:22778262
• Exhibits neuroprotective effects by regulating autophagic flux and acts as a potential tumor suppressor by stabilizing PHLPP1 in complex with WDR48. Related references: PMID:30266909, PMID:24145035

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human USP12, which may contribute to cross-reactivity with mouse and rat orthologs.
• For Western blot, a band around 43 kDa is expected; consider optimizing blocking conditions and antibody dilution for your specific sample.
• For ELISA, validate reactivity against the immunogen peptide or recombinant USP12 to confirm specificity and linearity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, from validated antibodies to ancillary reagents, supporting your diagnostic and research workflows. This anti-USP12 polyclonal antibody is part of our portfolio for deubiquitination and immune signaling studies. We also assist in sourcing specialized targets and custom bulk production to meet your assay development needs.