Anti-USP11 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P51784

Core Product Specifications and Parameters

Biological Background: USP11 Function and Localization

• USP11 is a deubiquitinating enzyme (ubiquitin carboxyl-terminal hydrolase) that removes ubiquitin from target proteins and polyubiquitin chains, preventing proteasomal degradation of its substrates. Related references: PMID:12084015 PMID:15314155 PMID:17897950
• The protease shows chain-linkage specificity, preferentially cleaving Lys-6 and Lys-63-linked ubiquitin chains, with much lower activity on Lys-48 linkages. Related references: PMID:24724799
• USP11 participates in NF-kappa-B activation pathways, contributing to inflammatory and immune signaling regulation. Related references: PMID:17897950 PMID:19874889
• It functions in DNA double-strand break repair, supporting genomic stability. Related references: PMID:15314155 PMID:20233726
• Acting as a chromatin regulator, USP11 associates with the Polycomb repressive complex PRC1-like and likely deubiquitinates its components, influencing gene silencing. Related references: PMID:20601937
• USP11 promotes cell proliferation by deubiquitinating and stabilizing phosphorylated E2F1, a key cell-cycle transcription factor. Related references: PMID:28992046
• The protein is mainly localized to the nucleus and cytoplasm, and it associates with chromosomes. Post-translational modifications include phosphorylation and acetylation, which may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 70–190 near the N-terminus, suggesting the antibody may recognize an epitope outside the catalytic domain; this could influence detection of truncated or cleaved forms.
• For Western blot, a band near 110 kDa is expected; when testing human, mouse, or rat lysates, confirm band specificity with appropriate controls due to possible cross-reactive signals.
• In immunofluorescence/immunocytochemistry, signal may be observed in both nucleus and cytoplasm, consistent with the reported subcellular distribution.
• For ELISA, the polyclonal nature permits capture/sandwich format optimization; consider pre-coating and detection antibody pairing empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing hub for IVD raw materials, streamlined to support diagnostic manufacturers from target discovery to assay development. For USP11-related projects, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate research and kit assembly. Whether you need rare-target reagents or large-volume procurement, CamelBio bridges your sourcing gaps with one-stop convenience.