Anti-USP11 Rabbit Monoclonal Antibody for WB and ELISA - P51784

Core Product Specifications and Parameters

Biological Background: USP11 Function and Localization

• Ubiquitin carboxyl-terminal hydrolase 11 (USP11) is a deubiquitinating enzyme that removes conjugated ubiquitin from target proteins and polyubiquitin chains, thereby regulating protein turnover and signaling. Related references: PMID:12084015, PMID:15314155, PMID:17897950
• USP11 inhibits the degradation of target proteins by the proteasome, contributing to stabilization of substrates. Related references: PMID:12084015
• It preferentially cleaves 'Lys-6' and 'Lys-63'-linked ubiquitin chains, has lower activity toward 'Lys-11' and 'Lys-33' linkages, and extremely low activity toward 'Lys-27', 'Lys-29', and 'Lys-48' linkages (in vitro). Related references: PMID:24724799
• USP11 plays a role in the regulation of pathways leading to NF-kappa-B activation, impacting immune and inflammatory responses. Related references: PMID:17897950, PMID:19874889
• It participates in DNA repair processes following double-stranded DNA breaks. Related references: PMID:15314155, PMID:20233726
• USP11 associates with the Polycomb group (PcG) multiprotein PRC1-like complex and may regulate chromatin structure by deubiquitinating components of this complex. Related references: PMID:20601937
• The enzyme promotes cell proliferation by deubiquitinating and stabilizing phosphorylated E2F1 transcription factor. Related references: PMID:28992046
• Subcellular localization includes the nucleus, cytoplasm, and chromosomes, consistent with its diverse functions.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to a synthetic peptide within amino acids 800–900 of human USP11. When designing experiments, consider that epitope accessibility may vary depending on protein conformation or post-translational modifications.
• For Western blotting (WB), USP11 has a predicted molecular weight of ~110 kDa. Use an appropriate gel percentage (e.g., 8–10% SDS-PAGE) and validate in samples known to express USP11, such as nuclear or cytoplasmic extracts from human, mouse, or rat cells.
• For ELISA applications, consider using recombinant USP11 protein or cell lysates as coating antigens, and optimize antibody dilution based on preliminary checkerboard titrations.
• Due to reported cross-reactivity with mouse and rat USP11, this antibody may be employed in rodent model studies; however, always confirm reactivity in the relevant sample system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, supporting manufacturers and laboratories from early-stage research to clinical application. For USP11 and related deubiquitination pathways, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Whether you need routine catalog items or assistance in sourcing rare-target raw materials, CamelBio is your reliable partner in accelerating diagnostic innovation.