Anti-USP1 Rabbit Polyclonal Antibody for WB - O94782

Core Product Specifications and Parameters

Biological Background: USP1 Function and Localization

• USP1 is a deubiquitinating enzyme (DUB) also known as Ubiquitin carboxyl-terminal hydrolase 1, Deubiquitinating enzyme 1 (hUBP), and Ubiquitin thioesterase 1.
• It negatively regulates the Fanconi anemia DNA repair pathway by deubiquitinating monoubiquitinated FANCD2. Related references: PMID:15694335
• USP1 also participates in PCNA-mediated translesion synthesis (TLS) by removing ubiquitin from monoubiquitinated PCNA. Related references: PMID:16531995, PMID:20147293
• The enzyme has low intrinsic catalytic activity and requires the interaction with its cofactor WDR48 for high deubiquitinase activity. Related references: PMID:18082604, PMID:26388029
• Subcellularly, USP1 localizes to the nucleus, consistent with its role in DNA repair processes.
• USP1 belongs to the peptidase C19 family of ubiquitin-specific proteases and is a thiol protease.
• It is implicated in the ubiquitin-proteasome system and is subject to autocatalytic cleavage and phosphorylation.
• Given its role in DNA damage response, USP1 expression is critical for genome maintenance.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-280); consider using this antibody to detect full-length USP1 or N-terminal fragments.
• For Western blot, optimize nuclear lysis and protein extraction conditions, as USP1 is a nuclear protein.
• Validate antibody specificity using positive and negative controls (e.g., USP1 knockout or siRNA knockdown samples).
• For ELISA assays, titrate the coating antigen and antibody dilution using recombinant USP1 or cellular lysates.
• Use appropriate anti-rabbit secondary antibodies for detection; ensure no cross-reactivity with target species reagents.
• In functional studies, co-expression of WDR48 may be required to observe USP1 enzymatic activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw-material sourcing, integrating validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-USP1 antibody supports research into DNA damage repair and ubiquitin-related pathways, with potential for oncology diagnostics. Leverage our supply capabilities to accelerate your assay development, from concept to clinic.