Anti-USO1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O60763

Core Product Specifications and Parameters

Biological Background: USO1 Function and Localization

• USO1 is a general vesicular transport factor p115, also designated protein USO1 homolog, transcytosis-associated protein (TAP), and vesicle-docking protein (VDP).
• It is essential for intercisternal transport within the Golgi stack, functioning in transcytotic fusion and binding of vesicles to the target membrane.
• The p115 protein likely serves as a vesicular anchor by interacting with the target membrane and maintaining close proximity between vesicular and target membranes.
• Subcellularly, USO1 localizes predominantly to the cytoplasm, cytosol, and Golgi apparatus membrane, consistent with its role in membrane trafficking.
• The protein undergoes post-translational modifications including phosphorylation and acetylation, which may regulate its activity or interactions.
• Structurally, p115 contains coiled-coil domains and repeat regions characteristic of tethering factors involved in vesicular transport.
• Its function is critical for ER-Golgi transport and general protein secretion, underscoring its importance in cellular logistics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 1100–1200) of human USO1; consider verifying antibody specificity using peptide competition assays or knockout cell lines to confirm target specificity.
• For Western blot (WB), a band at approximately 108 kDa is expected; optimize gel percentage and transfer conditions for high-molecular-weight proteins.
• In IHC-P and IF/ICC, antigen retrieval and permeabilization may be necessary due to the Golgi membrane association; validate fixation protocols for your sample type.
• As this is a polyclonal antibody, potential cross-reactivity with other vesicular tethering factors should be evaluated; testing in relevant cell or tissue models is recommended.
• Perform initial ELISA titration to determine optimal working concentrations for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and tailored rare target sourcing. This anti-USO1 rabbit polyclonal antibody supports research in Golgi trafficking and membrane transport, complementing our portfolio of reagents for cellular logistics studies. Let CamelBio streamline your transition from concept to clinic.