Anti-USO1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O60763

Core Product Specifications and Parameters

Biological Background: USO1 Function and Localization

• USO1 (also known as p115, TAP, VDP) is a general vesicular transport factor essential for intercisternal transport within the Golgi stack.
• The protein mediates transcytotic fusion and facilitates the tethering and docking of vesicles to target membranes, likely by holding vesicular and target membranes in proximity.
• USO1 belongs to the USO1 family and contains coiled-coil domains that are critical for its structural and functional roles.
• It localizes predominantly to the cytoplasm and the cytosolic face of the Golgi apparatus membrane.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics approaches.
• Alternative splicing generates multiple isoforms, which may contribute to functional diversity in vesicular trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 663-962 of human USO1, targeting a central region of the protein. This design may confer specificity for multiple isoforms.
• For Western blot (WB), consider using a positive control lysate from human or rat cell lines expressing USO1. The expected band is near 108 kDa, though isoforms may appear at different sizes.
• In immunofluorescence/ICC, permeabilization is required for cytoplasmic detection. Include a no-primary control to assess background.
• For ELISA, the antibody can be used as a capture or detection reagent. Validate with recombinant USO1 protein or peptide.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials like this USO1 polyclonal antibody, which supports research in Golgi dynamics and vesicular transport. Our inventory includes validated antibody pairs, bulk ancillary reagents, and sourcing capabilities for rare targets. Whether you need monoclonal or polyclonal antibodies, CamelBio can streamline your supply chain from concept to clinic.