Anti-USO1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O60763

Core Product Specifications and Parameters

Biological Background: USO1/p115 Function and Localization

• USO1 encodes the general vesicular transport factor p115, a homolog of the yeast USO1 protein, also designated transcytosis-associated protein (TAP) and vesicle-docking protein (VDP).
• p115 functions as a tethering factor essential for intra-Golgi transport, mediating intercisternal vesicle trafficking and transcytotic fusion events.
• It is proposed to act as a vesicular anchor, interacting with target membranes to maintain proximity between vesicular and target membranes during docking.
• Subcellularly, p115 localizes to the cytoplasm, cytosol, and Golgi apparatus membrane, consistent with its role in membrane trafficking.
• The protein contains coiled-coil domains and undergoes post-translational modifications including phosphorylation and acetylation, which may regulate its activity.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.
• UniProt keywords: 3D-structure, Acetylation, Alternative splicing, Coiled coil, Cytoplasm, ER-Golgi transport, Golgi apparatus, Membrane, Phosphoprotein, Protein transport, Proteomics identification, Reference proteome, Repeat, Transport.

Experimental Guidance and Technical Tips

• For western blotting, the predicted molecular weight is ~108 kDa; consider using a low-percentage SDS-PAGE gel and optimize transfer conditions for high-molecular-weight proteins. Validate with known positive controls.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; testing both citrate and EDTA-based buffers is recommended. Validate on tissue sections with documented USO1 expression.
• For immunofluorescence/ICC, include a permeabilization step to allow nuclear and Golgi access. Co-stain with a Golgi marker (e.g., GM130) to confirm expected localization.
• The immunogen is an N-terminal peptide (aa 1-100); confirm that the epitope is present in your target samples, especially if using truncated constructs.
• Cross-reactivity with mouse and rat USO1 is indicated; verify sequence alignment of the immunogen region to ensure detection if using these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with validated IVD raw materials, including optimized monoclonal/polyclonal antibodies, antibody pairs, and bulk ancillary reagents. The USO1/p115 antibody supports studies on Golgi transport and membrane trafficking, and our platform can help source rare-target reagents or provide customized supply solutions to advance your projects from concept to clinic.