Anti-USF2 Rabbit Polyclonal Antibody for WB, ELISA - Q15853

Core Product Specifications and Parameters

Biological Background: USF2 Function and Localization

• Upstream stimulatory factor 2 (USF2) is a member of the class B basic helix-loop-helix (bHLH) transcription factor family, also known as FOS-interacting protein (FIP) or bHLHb12.
• USF2 functions as a transcription factor by binding to the symmetrical E-box DNA sequence (5'-CACGTG-3') present in many viral and cellular promoters.
• Through E-box binding, USF2 regulates the expression of genes involved in diverse processes such as cell cycle control, metabolism, and stress responses.
• USF2 is localized predominantly in the nucleus, consistent with its role in transcriptional regulation.
• Expression of USF2 is ubiquitous across human tissues, indicating broad regulatory functions.
• Alternative splicing of the USF2 gene generates multiple isoforms, which may exhibit distinct transcriptional activities or target gene specificities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 101–200) common to multiple USF2 isoforms; the antibody may therefore detect several splice variants.
• For Western blot, a predominant band around 37 kDa is expected; consider running appropriate controls and validating band specificity in the relevant sample system.
• The antibody is reported to cross-react with mouse and rat USF2; confirm reactivity in your specific lysates or tissue types.
• In ELISA, test the antibody as either capture or detection reagent after optimizing coating conditions and antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering a broad portfolio of validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents for diagnostic assay development. Our USF2 rabbit polyclonal antibody supports research into transcriptional regulation and E-box-mediated gene expression. With deep expertise in rare target sourcing and end-to-end supply solutions, CamelBio helps accelerate your project from concept to clinic.