Anti-USF1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P22415

Core Product Specifications and Parameters

Biological Background: USF1 Function and Localization

• USF1 (Upstream stimulatory factor 1) is a member of the basic helix-loop-helix (bHLH) family of transcription factors, also known as bHLHb11.
• It functions by binding to symmetrical E-box sequences (5'-CACGTG-3') in the promoters of various viral and cellular genes, regulating their transcription.
• The protein localizes exclusively to the nucleus, consistent with its role in transcriptional control.
• Alternative splicing results in multiple isoforms, as indicated by UniProt.
• Post-translational modifications include Ubl conjugation and isopeptide bond formation, which may modulate its activity or stability.
• Other aliases include Major late transcription factor 1 (MLTF1) and FCHL1, reflecting its involvement in lipid metabolism and other pathways.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment covering amino acids 1-310 of human USF1 (NP_009053.1). This N-terminal region includes the transactivation domain and may provide specificity for detecting the target.
• For Western blotting, a band around 34 kDa is expected in human, mouse, and rat samples. Pre-validate in your specific experimental system.
• In immunohistochemistry (IHC-P), antigen retrieval conditions and dilution should be optimized with appropriate controls for each tissue type.
• As a polyclonal antibody, it may recognize multiple isoforms from alternative splicing; confirm specificity using appropriate positive and negative controls.
• Given the antibody's reactivity with mouse and rat USF1, it may be suitable for cross-species studies; however, sequence alignment and empirical validation are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for assay development. For transcription factor targets like USF1, we offer reliable sourcing solutions to support your immunoassay and research needs.