Anti-USF1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P22415

Core Product Specifications and Parameters

Biological Background: USF1 Function and Localization

• USF1 is a transcription factor that specifically binds to the E-box sequence (5'-CACGTG-3'), which is present in numerous viral and cellular promoters.
• It belongs to the class B basic helix-loop-helix (bHLH) transcription factor family, also designated bHLHb11 or major late transcription factor 1 (MLTF1).
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• USF1 undergoes alternative splicing, generating multiple isoforms that may exhibit distinct transcriptional activities.
• Post-translational modifications include isopeptide bond formation and ubiquitin conjugation (Ubl conjugation), as indicated by UniProt keywords.
• With a calculated molecular weight of approximately 34 kDa (UniProt: 33538 Da), USF1 is a well-characterized reference proteome member identified in several proteomics studies.
• The protein plays a central role in transcription regulation, as evidenced by direct protein sequencing and 3D structural characterization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-310, encompassing the N-terminal region of USF1; consider this when designing epitope-specific assays.
• For Western blot (WB), the antibody detects a band around 34 kDa; validate with appropriate positive and negative controls (e.g., USF1 knockdown or knockout lysates if available).
• Cross-reactivity with mouse and rat USF1 suggests conserved epitopes; however, we advise sequence alignment of your target species to confirm recognition.
• In immunoprecipitation (IF-P) and immunohistochemistry (IHC-P), optimize blocking and permeabilization steps to ensure nuclear staining.
• For ELISA, the antibody may be used for capture or detection; titrate to achieve optimal signal-to-noise ratio.
• The KD validation indicates the antibody's suitability for confirming USF1 knockdown studies; consider verifying with independent siRNA or CRISPR-mediated depletion.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive one-stop sourcing solution for IVD raw materials, including high-quality antibodies like this anti-USF1 monoclonal antibody. As your B2B partner, we offer validated antibody pairs, bulk ancillary reagents, and rare target raw-material procurement to support transcription-related assay development and clinical workflows. Let us bridge your sourcing needs from concept to clinic.