Anti-USF1 Rabbit Monoclonal Antibody for WB and ELISA - P22415

Core Product Specifications and Parameters

Biological Background: USF1 Function and Localization

• USF1 (Upstream stimulatory factor 1) is a transcription factor also known as Class B basic helix-loop-helix protein 11 (bHLHb11) or Major late transcription factor 1.
• It binds to symmetrical E-box DNA sequences (5'-CACGTG-3') found in various viral and cellular promoters, regulating transcription.
• The protein contains a basic helix-loop-helix (bHLH) domain, enabling DNA binding and dimerization.
• USF1 localizes to the nucleus, consistent with its role in transcriptional regulation.
• Alternative splicing generates multiple isoforms of USF1, as indicated by the "Alternative splicing" keyword.
• Post-translational modifications include isopeptide bond formation and ubiquitin-like conjugation (Ubl conjugation), which may modulate its activity or stability.
• USF1 is a member of the basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factor family, playing roles in diverse cellular processes.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 100-200) lies within a region that may be conserved; based on reactivity with both human and mouse USF1, consider validating in the relevant sample system.
• For Western blot, the predicted molecular weight is ~34 kDa; choose an appropriate gel percentage and transfer conditions to resolve this region.
• For ELISA, specific conditions (coating antigen, antibody dilution) should be optimized for the assay format to ensure reliable detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, streamlining development from concept to clinic. This USF1 antibody supports transcriptional regulation studies and assay development. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to meet your entire project needs.