Anti-Uroplakin III/UPK3A Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q9JKX8

Core Product Specifications and Parameters

Biological Background: Uroplakin-3a Function and Localization

• Uroplakin-3a (UPK3A), also known as Uroplakin III (UPIII), is a transmembrane glycoprotein specifically expressed in terminally differentiated urothelial cells.
• It is an integral component of the asymmetric unit membrane (AUM), a highly specialized biomembrane that lines the apical surface of the bladder urothelium.
• UPK3A is involved in mediating interactions between the AUM and the cytoskeleton, contributing to the structural integrity and mechanical stability of the urothelial plaque.
• It participates in the formation of the urothelial glycocalyx, a protective layer that may inhibit bacterial adherence to the bladder surface.
• The protein localizes predominantly to the endoplasmic reticulum membrane, consistent with its role in the biosynthesis and assembly of urothelial plaques.

Experimental Guidance and Technical Tips

• For western blotting, anticipate a specific band at approximately 31 kDa in mouse bladder or urothelial tissue lysates; include appropriate positive and negative controls to confirm antibody specificity.
• When performing immunohistochemistry on formalin-fixed paraffin-embedded (FFPE) sections, optimize heat-induced antigen retrieval protocols (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) to enhance signal detection.
• In immunofluorescence applications, permeabilization with a mild detergent (e.g., 0.1% Triton X-100) may be necessary to access the intracellular endoplasmic reticulum membrane localization of UPK3A.
• For ELISA, consider using recombinant UPK3A protein or peptide as coating antigen and titrate the antibody to determine the optimal working concentration.
• Cross-reactivity with human and rat UPK3A has been reported; however, verify performance in your experimental system using species-appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials like this anti-UPK3A polyclonal antibody, supporting studies on urothelial differentiation and bladder physiology. Our comprehensive sourcing capabilities extend to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Whether you need rare-target antibodies or scalable production, CamelBio bridges the gap from concept to clinic with reliable and efficient supply solutions.