Anti-UROD Polyclonal Antibody for WB, ELISA - P06132

Core Product Specifications and Parameters

Biological Background: UROD Function and Localization

• UROD encodes uroporphyrinogen decarboxylase, a key enzyme localized to the cytoplasm and cytosol.
• It catalyzes the sequential decarboxylation of the four acetate side chains of uroporphyrinogen III to form coproporphyrinogen III, an essential step in heme biosynthesis (PubMed:11069625, PubMed:11719352, PubMed:14633982). Related references: PMID:11069625 PMID:11719352 PMID:14633982
• The enzyme also acts on uroporphyrinogen I and, in vitro, decarboxylates pentacarboxylate porphyrinogen I (PubMed:12071824). Related references: PMID:12071824
• UROD belongs to the porphyrin biosynthesis pathway and is annotated with keywords including Acetylation, Decarboxylase, Lyase, and Disease variant, indicating roles in post-translational modification and genetic disorders.
• The protein has a calculated molecular weight of approximately 41 kDa (40787 Da), consistent with its electrophoretic mobility.
• Three-dimensional structural data are available for UROD, revealing insights into its catalytic mechanism.
• It is a member of the reference proteome and has been identified by mass spectrometry-based proteomics.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant human UROD protein (residues 1–367), which may facilitate recognition of both linear and conformational epitopes.
• For Western blot (WB) analysis, a band at ~41 kDa is anticipated; consider optimizing blocking and antibody dilution conditions for your sample type.
• When using this antibody in ELISA, perform checkerboard titrations to determine optimal coating antigen and antibody concentrations.
• The reported cross-reactivity with mouse UROD suggests utility in rodent models; however, validation in the specific experimental context is recommended.
• If high background is observed in WB, pre-adsorption against a negative control lysate may improve specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, providing researchers and manufacturers with reliable antibodies and recombinant proteins for diagnostic assay development. This anti-UROD polyclonal antibody supports investigations into heme biosynthesis and related disorders, and complements our extensive portfolio of metabolism-related reagents. For bulk supply, custom antibody generation, or specialized raw material needs, CamelBio offers integrated solutions tailored to accelerate your product pipeline.