Anti-URM1 Polyclonal Antibody for WB, ELISA - Q9BTM9

Core Product Specifications and Parameters

Biological Background: URM1 Function and Localization

• URM1 acts as a sulfur carrier for 2-thiolation of mcm5S2U at wobble positions of tRNALys, tRNAGlu, and tRNAGln, requiring thiocarboxylation at its C-terminus by MOCS3. Related references: PMID:19017811 PMID:22453920
• It also functions as a ubiquitin-like modifier (UBL), forming isopeptide bonds with lysine residues on target proteins such as MOCS3, ATPBD3, CTU2, USP15, and CAS. Related references: PMID:21209336
• The thiocarboxylated form serves as the conjugation substrate, and oxidative stress specifically enhances UBL-protein conjugate formation. Related references: PMID:21209336
• URM1 is found in the cytoplasm, cytosol, and nucleus.
• The protein undergoes alternative splicing and is subject to phosphorylation and thiocarboxylation.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-63) of human URM1; consider this when interpreting signals, as isoform-specific recognition may vary.
• For Western blot detection of the 11 kDa protein, using a high-percentage gel or optimized transfer conditions is recommended to ensure retention.
• The antibody is validated for WB and ELISA; optimal working dilutions should be determined empirically for your specific experimental setup.
• Cross-reactivity is reported for mouse and rat; however, end-user validation in these species is advised.
• ELISA performance may require optimization of coating antigen concentration and antibody incubation parameters.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-URM1 polyclonal antibody supports research in tRNA modification and ubiquitin-like pathways, offering reliable reagent sourcing for pathway-focused studies. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and production needs.