Anti-Uridine-cytidine kinase 2 Rabbit Polyclonal Antibody for WB, ELISA - Q9BZX2

Core Product Specifications and Parameters

Biological Background: UCK2 Function and Localization

• UCK2 phosphorylates uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), playing a key role in pyrimidine nucleoside salvage. Related references: PMID:11306702, PMID:11494055, PMID:35583288
• The enzyme specifically does not recognize deoxyribonucleosides or purine ribonucleosides as substrates, and can utilize either ATP or GTP as the phosphate donor. Related references: PMID:11306702
• UCK2 also exhibits broad activity toward various pyrimidine nucleoside analogs, including 6-azauridine, 5-fluorouridine, 4-thiouridine, and 5-fluorocytidine, demonstrating its potential role in activating anticancer and antiviral prodrugs. Related references: PMID:11306702
• Tissue expression of UCK2 is reported to be testis-specific or placenta-specific, depending on the study, suggesting a specialized role in these tissues. Related references: PMID:8812458, PMID:11306702
• Post-translational modifications include acetylation and phosphorylation, and the gene undergoes alternative splicing, yielding multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human UCK2 protein (aa 1-261), which is highly conserved; cross-reactivity with mouse UCK2 is expected, but validation in the relevant sample system is recommended.
• For Western blot (WB), a band at approximately 29 kDa is anticipated based on the predicted molecular weight; however, due to potential post-translational modifications, migration may vary.
• In ELISA applications, consider pairing with a suitable detection antibody and optimizing coating conditions.
• Since UCK2 expression may be tissue-specific, appropriate positive control lysates (e.g., testis or placenta) should be included.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, optimized reagents, and technical services to accelerate diagnostic development. For nucleoside kinase research, we supply high-quality polyclonal antibodies like this anti-UCK2 antibody, along with matched detection reagents and bulk ancillary materials. From concept to clinic, CamelBio supports your assay development with reliable, scalable solutions.