Anti-UQCRQ Polyclonal Antibody for WB, ELISA - O14949

Core Product Specifications and Parameters

Biological Background: UQCRQ Function and Localization

• UQCRQ encodes cytochrome b-c1 complex subunit 8, also known as complex III subunit VIII, ubiquinol-cytochrome c reductase complex 9.5 kDa protein, or ubiquinone-binding protein QP-C.
• It is a key component of the mitochondrial ubiquinol-cytochrome c oxidoreductase (complex III), which catalyzes electron transfer from ubiquinol to cytochrome c in the electron transport chain.
• Complex III contributes to the proton gradient across the inner membrane via the Q cycle, taking two protons from the matrix and releasing four into the intermembrane space.
• UQCRQ is located in the mitochondrial inner membrane and spans the membrane as a transmembrane helix.
• Post-translational modifications include acetylation and phosphorylation; it has been identified by proteomics and is a member of the reference proteome.
• Mutations in UQCRQ are associated with primary mitochondrial disease, highlighting its essential role in oxidative phosphorylation.
• The molecular weight is approximately 9.9 kDa, consistent with the observed 10 kDa band.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1–82 amino acids of human UQCRQ; this region includes the mitochondrial targeting sequence and part of the mature protein.
• For Western blot, consider using mitochondrial-enriched lysates to enhance signal, and expect a band at ~10 kDa under reducing conditions.
• This antibody has been tested in WB and ELISA; for other applications like ICC or IP, independent validation is recommended.
• When working across species, confirm sequence homology; cross-reactivity is demonstrated for human and mouse samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, clinical laboratories, and research institutes with reliable access to IVD raw materials, technical services, and consulting. This UQCRQ rabbit polyclonal antibody supports respiratory chain and mitochondrial disease research. We offer validated antibody pairs, optimized monoclonal/polyclonal reagents, and ancillary bulk materials to streamline your assay development from concept to clinic.