Anti-UQCRFS1 (Rieske Iron-Sulfur Protein) Polyclonal Antibody for WB, IHC-P, ELISA - P47985

Core Product Specifications and Parameters

Biological Background: UQCRFS1 Function and Localization

• UQCRFS1, also known as Rieske iron-sulfur protein (RISP), is a core subunit of the mitochondrial cytochrome b-c1 complex (Complex III) of the electron transport chain.
• It contains a [2Fe-2S] cluster and facilitates electron transfer from ubiquinol to cytochrome c1, contributing to the proton gradient via the Q cycle. Related references: PMID:31883641
• UQCRFS1 undergoes proteolytic processing upon insertion into the Complex III dimer, releasing a fragment called subunit 9, which is essential for proper complex assembly. Related references: PMID:28673544
• Incorporation of UQCRFS1 is the penultimate step in Complex III assembly; unprocessed fragments can disrupt complex function. Related references: PMID:28673544
• The protein is located in the mitochondrial inner membrane.
• Key UniProt keywords include 2Fe-2S, iron-sulfur, electron transport, respiratory chain, and primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 141–274 of human UQCRFS1, a region within the mature protein; the antibody is predicted to detect processed forms.
• In Western blot, a predominant band near 30 kDa is expected; additional bands representing proteolytic fragments may also be observed. Consider using mitochondrial-enriched lysates for enhanced signal.
• For IHC-P, antigen retrieval may be required; co-staining with a mitochondrial marker can help confirm localization.
• Cross-reactivity with mouse and rat is based on sequence homology; validation in the relevant species and sample type is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your mitochondrial respiratory chain research as a one-stop IVD raw-material partner. We provide this UQCRFS1 antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—streamlining your development from concept to clinic.