Anti-UQCRFS1 Polyclonal Antibody for WB, IHC-P, ELISA - P47985

Core Product Specifications and Parameters

Biological Background: UQCRFS1 Function and Localization

• UQCRFS1 encodes the Rieske iron-sulfur protein, a catalytic subunit of the mitochondrial ubiquinol-cytochrome c oxidoreductase (complex III). Complex III is a key component of the electron transport chain, transferring electrons from ubiquinol to cytochrome c and contributing to the proton gradient for ATP synthesis. Related references: PMID:31883641
• The protein contains a [2Fe-2S] cluster essential for its electron transfer function and cycles between conformational states during the Q cycle.
• UQCRFS1 is proteolytically processed upon incorporation into the complex III dimer, cleaving its mitochondrial targeting sequence; this processing is required for proper assembly and function. Related references: PMID:28673544
• The protein localizes to the mitochondrial inner membrane, consistent with its role in oxidative phosphorylation.
• Known aliases include RIP1, RIS1, RISP, UQCR5, and MC3DN10, reflecting its functional and clinical associations.
• Keywords: 2Fe-2S, electron transport, respiratory chain, iron-sulfur, mitochondrial inner membrane, translocase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (amino acids 79-274) of human UQCRFS1, which may recognize both full-length and processed forms; consider validation in the relevant sample system.
• For Western blot (WB), a band around 30 kDa is expected; cross-reactivity with mouse should be confirmed with appropriate controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution using appropriate positive and negative controls.
• For ELISA, ensure coating conditions and detection limits are established for the intended target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibodies and ancillary reagents. This UQCRFS1 polyclonal antibody is ideal for mitochondrial complex III research, and CamelBio can assist with sourcing additional related reagents such as matched antibody pairs or recombinant proteins to streamline your assay development.