Anti-UQCRC2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P22695

Core Product Specifications and Parameters

Biological Background: UQCRC2 Function and Localization

• UQCRC2, also known as Core protein II, is a subunit of the mitochondrial ubiquinol-cytochrome c oxidoreductase (complex III; cytochrome b-c1 complex), which is a critical component of the electron transport chain driving oxidative phosphorylation.
• Complex III catalyzes the transfer of electrons from ubiquinol to cytochrome c, coupled with proton translocation across the mitochondrial inner membrane via the Q cycle, contributing to the proton gradient used for ATP synthesis.
• The core subunits UQCRC1 and UQCRC2 are homologous to the alpha and beta subunits of mitochondrial-processing peptidase (MPP), respectively, and may retain processing activity, potentially involved in maturation of the Rieske iron-sulfur protein (UQCRFS1) upon assembly into complex III.
• UQCRC2 is located in the mitochondrial inner membrane, consistent with its role in the respiratory chain.
• Mutations in UQCRC2 are associated with mitochondrial complex III deficiency, nuclear type 5 (MC3DN5), a disorder characterized by lactic acidosis, hypoglycemia, and encephalopathy.
• Post-translational modifications include acetylation, which may regulate enzyme activity or stability.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 48 kDa is expected; optimize primary antibody concentration and detection conditions for your specific sample and protocol.
• In IHC-P, consider using heat-induced epitope retrieval and test a range of antibody dilutions to achieve optimal specific staining.
• The immunogen sequence (aa 250–350) lies within a conserved region, supporting the observed cross-reactivity with mouse and rat; validate in the relevant sample system.
• As a monoclonal antibody, it offers consistent lot-to-lot performance; confirm suitability for your intended application by initial pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and research labs, providing a comprehensive portfolio that includes validated antibodies targeting mitochondrial respiratory chain components like UQCRC2. Our offerings extend to optimized antibody pairs for ELISA, monoclonal/polyclonal antibodies for various applications, and bulk ancillary reagents, all backed by efficient sourcing to support your projects from concept to clinic. Whether you need rare target reagents or large-scale production materials, CamelBio serves as your reliable sourcing bridge.