Anti-UQCRC2 Polyclonal Antibody (Rabbit) for WB, IHC-P, IF/ICC, IP, ELISA - P22695

Core Product Specifications and Parameters

Biological Background: UQCRC2 Function and Localization

• UQCRC2, also known as Core protein II or QCR2, is a core subunit of the mitochondrial cytochrome b-c1 complex (complex III), which is essential for oxidative phosphorylation and ATP production.
• The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, coupling this redox reaction to proton translocation across the inner mitochondrial membrane via the Q cycle.
• UQCRC2 is homologous to the alpha subunit of mitochondrial-processing peptidase (MPP) and may participate in the processing of the Rieske protein (UQCRFS1) into its mature form within complex III.
• Localized to the mitochondrial inner membrane, UQCRC2 faces the matrix side and contributes to the structural integrity and assembly of the respiratory chain supercomplex.
• Post-translational modifications include acetylation, which potentially regulates complex III activity or stability.
• Mutations in UQCRC2 are associated with mitochondrial complex III deficiency (MC3DN5), a primary mitochondrial disease presenting with severe neurological and metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fragment (aa 224-453) of human UQCRC2, which is predicted to confer high specificity for the target protein.
• For Western blotting, a band at approximately 48 kDa is expected in mitochondrial-enriched lysates from human, mouse, or rat tissues. Optimization of lysis conditions may improve detection.
• Immunoprecipitation and immunofluorescence applications require careful titration of the antibody concentration; consider including positive and negative controls to assess specificity.
• The antibody is suitable for ELISA; direct coating of the recombinant immunogen or peptide can serve as a positive binding control.
• For IHC-P and IF/ICC, antigen retrieval methods and permeabilization steps should be optimized for the target tissue or cell type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a reliable one-stop sourcing channel for IVD raw materials. This UQCRC2 polyclonal antibody supports mitochondrial dysfunction and metabolic disease research, and we offer complementary products such as validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For unique or rare target sourcing, our global network ensures timely access to specialized raw materials, accelerating your project from concept to clinic.